Green tea component EGCG, insulin and IGF-1 promote nuclear efflux of atrophy-associated transcription factor Foxo1 in skeletal muscle fibers

The Journal of Nutritional Biochemistry
Robert J WimmerMartin F Schneider

Abstract

Prevention and slowing of skeletal muscle atrophy with nutritional approaches offers the potential to provide far-reaching improvements in the quality of life for our increasingly aging population. Here we show that polyphenol flavonoid epigallocatechin 3-gallate (EGCG), found in the popular beverage green tea (Camellia sinensis), demonstrates similar effects to the endogenous hormones insulin-like growth factor 1 (IGF-1) and insulin in the ability to suppress action of the atrophy-promoting transcription factor Foxo1 through a net translocation of Foxo1 out of the nucleus as monitored by nucleo-cytoplasmic movement of Foxo1-green fluorescent protein (GFP) in live skeletal muscle fibers. Foxo1-GFP nuclear efflux is rapid in IGF-1 or insulin, but delayed by an additional 30 min for EGCG. Once activated, kinetic analysis with a simple mathematical model shows EGCG, IGF-1 and insulin all produce similar apparent rate constants for Foxo1-GFP unidirectional nuclear influx and efflux. Interestingly, EGCG appears to have its effect at least partially via parallel signaling pathways that are independent of IGF-1's (and insulin's) downstream PI3K/Akt/Foxo1 signaling axis. Using the live fiber model system, we also determine the dose-res...Continue Reading

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Citations

May 23, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Qiu-Yue FuYue-Rong Liang
Mar 28, 2020·Antioxidants & Redox Signaling·Li Li JiChounghun Kang
Feb 7, 2021·The Journal of Biological Chemistry·Rui CaiWeijun Pang
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Takeshi NikawaIori Sakakibara

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