Green Tea Extract Ameliorates Ischemia-Induced Retinal Ganglion Cell Degeneration in Rats

Oxidative Medicine and Cellular Longevity
Yaping YangTsz Kin Ng

Abstract

Oxidative stress induced by reduced blood circulation is a critical pathological damage to retinal ganglion cells (RGCs) in glaucoma. We previously showed that green tea extract (GTE) and its catechin constituents alleviate sodium iodate-induced retinal degeneration in rats. Here, we investigated the therapeutic effect of GTE on ischemia-induced RGC degeneration in rats. RGC degeneration was induced by ischemic reperfusion in adult Fischer F344 rats. Green tea extract (Theaphenon E) was intragastrically administered 4 times within 48 hours after ischemia. RGC survival, pupillary light reflex, expressions of cell apoptosis, oxidative stress, and inflammation-related proteins were studied. Ischemic reperfusion significantly induced apoptotic RGCs, RGC loss, and larger constricted pupil area compared to the untreated normal rats. Expressions of activated caspase-3 and caspase-8, Sod2, and inflammation-related proteins as well as p38 phosphorylation were significantly upregulated in the ischemia-injured rats. Compared to the saline-fed ischemic rats, significantly higher number of surviving RGCs, less apoptotic RGCs, and smaller constricted pupil area were observed in the GTE-fed ischemic rats. GTE also reduced the increased protei...Continue Reading

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Citations

Mar 4, 2020·Biology·Anna PeresypkinaVladimir Pokrovskii
Oct 4, 2020·European Journal of Ophthalmology·Sayena JabbehdariThasarat Sutabutr Vajaranant
Oct 22, 2020·Nutrients·Annagrazia AdornettoRossella Russo
Oct 28, 2020·Antioxidants·Jose Javier Garcia-MedinaMonica Del-Rio-Vellosillo

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Methods Mentioned

BETA
protein assay

Software Mentioned

SPSS Statistics
ImageJ
SPSS

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