Green Tea Extract Induces the Resistance of Caenorhabditis elegans against Oxidative Stress

Antioxidants
S Abbas, Michael Wink

Abstract

Epidemiological studies on the effects of green tea consumption (Camellia sinensis) have demonstrated a reduction for the risk of age-related diseases. The investigation of the in vivo and in vitro antioxidant properties of an aqueous extract of green tea (GTE) was the aim of the current study. 2,2-Diphenyl-1-picrylhydrazyl (DPPH(•)) and superoxide anion radical (O₂(•-)) assays were used to estimate the GTE antioxidant activity. To investigate the protective effects of GTE against oxidative stress, wild-type N2 and transgenic strains (TJ374, hsp-16.2/GFP) of the model organism, Caenorhabditis elegans (C. elegans), were chosen. In the current study, the following catechins were identified by LC/ESI-MS: catechin, epicatechin, epicatechin gallate, gallocatechin, epigallocatechin and epigallocatechin gallate. GTE exhibited a free radical scavenging activity of DPPH(•) and O₂(•-) with IC50 8.37 and 91.34 µg/mL, respectively. In the C. elegans strain (TJ374, hsp-16.2/GFP), the expression of hsp-16.2/GFP was induced by a nonlethal dose of juglone, and the fluorescence density of hsp-16.2/GFP was measured. The hsp-16.2/GFP was reduced by 68.43% in the worms pretreated with 100 µg/mL GTE. N2 worms pretreated with 100 µg/mL GTE exhibited...Continue Reading

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Citations

Sep 9, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mansour SobehMichael Wink
Dec 1, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mansour SobehMichael Wink
Mar 3, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Herbenya PeixotoMichael Wink
Sep 24, 2019·Journal of Medicinal Food·Inés ReigadaCarlota Gómez Rincón
Mar 14, 2020·Journal of the Science of Food and Agriculture·Irene ZorraquínBegoña Bartolomé
Jul 10, 2019·BMC Complementary and Alternative Medicine·Panthakarn RangsinthTewin Tencomnao
May 28, 2019·Antioxidants·María Pilar de TorreJosé Luis Vizmanos
Feb 25, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Ki-Bae HongYooheon Park
Jul 31, 2021·Frontiers in Bioengineering and Biotechnology·Limin LiuNan Liu

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