Green tea polyphenol (-)-epigallocatechin gallate blocks epithelial barrier dysfunction provoked by IFN-gamma but not by IL-4

American Journal of Physiology. Gastrointestinal and Liver Physiology
James L WatsonD M McKay

Abstract

A characteristic of many enteropathies is increased epithelial permeability, a potentially pathophysiological event that can be evoked by T helper (Th)-1 (i.e., IFN-gamma) and Th2 (i.e., IL-4) cytokines and bacterial infection [e.g., enteropathogenic Escherichia coli (EPEC)]. The green tea polyphenol (-)-epigallocatechin gallate (EGCG) has immunosuppressive properties, and we hypothesized that it would ameliorate the increased epithelial permeability induced by IFN-gamma, IL-4, and/or EPEC. EGCG, but not the related epigallocatechin, completely prevented the increase in epithelial (i.e., T84 cell monolayer) permeability caused by IFN-gamma exposure as gauged by transepithelial resistance and horseradish peroxidase flux; EGCG did not alleviate the barrier disruption induced by IL-4 or EPEC. IFN-gamma-treated T84 and THP-1 (monocytic cell line) cells displayed STAT1 activation (tyrosine phosphorylation on Western blot analysis, DNA binding on EMSA) and upregulation of interferon response factor-1 mRNA, a STAT1-dependent gene. All three events were inhibited by EGCG pretreatment. Aurintricarboxylic acid also blocked IFN-gamma-induced STAT1 activation, but it did not prevent the increase in epithelial permeability. Additionally, ph...Continue Reading

References

Feb 1, 1989·The Journal of Clinical Investigation·J L Madara, J Stafford
Oct 1, 1983·The Journal of Pediatrics·S TsuchiyaK Tada
Feb 1, 1996·The American Journal of Physiology·D M McKayM H Perdue
Apr 1, 1996·The American Journal of Physiology·D J PhilpottM H Perdue
Jul 1, 1997·Gastroenterology·P DesreumauxJ F Colombel
Jul 11, 1998·The Journal of Biological Chemistry·M F FavataJ M Trzaskos
May 18, 1999·The American Journal of Physiology·M C BerinM H Perdue
Jun 2, 2000·Gastroenterology·T KinugasaH C Reinecker
Oct 29, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·D M McKayC D Richards
May 10, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M MenegazziH Suzuki
Aug 21, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J Y ChungC S Yang
Mar 22, 2002·Nature Reviews. Immunology·C Nagler-Anderson
Apr 4, 2002·Trends in Immunology·Chilakamarti V RamanaGeorge R Stark
May 2, 2002·Cell·John J O'SheaRobert D Schreiber
Oct 22, 2002·American Journal of Physiology. Cell Physiology·Kathryn HoweDerek M McKay
Jul 24, 2003·Nature Reviews. Immunology·Gerd Bouma, Warren Strober
Nov 25, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Matthias BruewerAsma Nusrat
May 15, 2004·JPEN. Journal of Parenteral and Enteral Nutrition·David L SigaletJon B Meddings

❮ Previous
Next ❯

Citations

Aug 6, 2009·The Journal of Biological Chemistry·Michael ScharlDeclan F McCole
Feb 7, 2009·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Cécile BeaurepaireDerek M McKay
Jan 9, 2009·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Michel A BoivinThomas Y Ma
Nov 19, 2008·Journal of Neuroinflammation·Brian GiuntaJun Tan
Mar 11, 2009·Frontiers in Bioscience (Landmark Edition)·Rana Al-SadiThomas Ma
May 25, 2011·World Journal of Gastrointestinal Pathophysiology·Hirotada AkihoKazuhiko Nakamura
Jul 12, 2012·Cellular and Molecular Life Sciences : CMLS·Takuya Suzuki
Dec 24, 2015·Frontiers in Immunology·Stefania De SantisMarcello Chieppa
Mar 24, 2016·Critical Reviews in Food Science and Nutrition·Guan YangMei-Jun Zhu
Jun 6, 2006·Biochemical Pharmacology·Sanghamitra BandyopadhyayNaibedya Chattopadhyay
Feb 22, 2017·Molecular Nutrition & Food Research·Katherine Gil-CardosoXimena Terra
Feb 16, 2011·Laboratory Investigation; a Journal of Technical Methods and Pathology·David SmythDerek M McKay
Dec 19, 2018·International Journal of Environmental Research and Public Health·Fatma N HamedRachid Tazi-Ahnini
Apr 10, 2010·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·A V Keita, J D Söderholm
Jan 12, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Kimberley LewisDerek M McKay
Oct 22, 2016·European Journal of Gastroenterology & Hepatology·Roberta E RossiMartyn E Caplin
Mar 29, 2020·Animal Science Journal = Nihon Chikusan Gakkaihō·Takuya Suzuki
Jun 23, 2009·Annals of the New York Academy of Sciences·Maren AmashehJörg-Dieter Schulzke
Jun 27, 2012·Annals of the New York Academy of Sciences·Maren AmashehJörg-Dieter Schulzke
Mar 25, 2011·The Journal of Nutrition·Dulantha UlluwishewaNicole C Roy
Jun 1, 2008·Comprehensive Reviews in Food Science and Food Safety·M G SajilataR S Singhal
Jan 11, 2018·Molecular Aspects of Medicine·P I OteizaD H Taft
Feb 25, 2017·Journal of Natural Products·Sandro LuescherVeronika Butterweck
Jul 3, 2019·Journal of Agricultural and Food Chemistry·Stefano BernardiPatrizia Riso
Dec 21, 2010·The Journal of Nutritional Biochemistry·Takuya Suzuki, Hiroshi Hara

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.