Green tea polyphenol epigallocatechin-3-gallate and cranberry proanthocyanidins act in synergy with cathelicidin (LL-37) to reduce the LPS-induced inflammatory response in a three-dimensional co-culture model of gingival epithelial cells and fibroblasts

Archives of Oral Biology
Telma Blanca Lombardo BedranDaniel Grenier

Abstract

The human antimicrobial peptide cathelicidin (LL-37) possesses anti-inflammatory properties that may contribute to attenuating the inflammatory process associated with chronic periodontitis. Plant polyphenols, including those from cranberry and green tea, have been reported to reduce inflammatory cytokine secretion by host cells. In the present study, we hypothesized that A-type cranberry proanthocyanidins (AC-PACs) and green tea epigallocatechin-3-gallate (EGCG) act in synergy with LL-37 to reduce the secretion of inflammatory mediators by oral mucosal cells. A three-dimensional (3D) co-culture model of gingival epithelial cells and fibroblasts treated with non-cytotoxic concentrations of AC-PACs (25 and 50 μg/ml), EGCG (1 and 5 μg/ml), and LL-37 (0.1 and 0.2 μM) individually and in combination (AC-PACs+LL-37 and EGCG+LL-37) were stimulated with Aggregatibacter actinomycetemcomitans lipopolysaccharide (LPS). Multiplex ELISA assays were used to quantify the secretion of 54 host factors, including chemokines, cytokines, growth factors, matrix metalloproteinases (MMPs), and tissue inhibitors of metalloproteinases (TIMPs). LL-37, AC-PACs, and EGCG, individually or in combination, had no effect on the regulation of MMP and TIMP sec...Continue Reading

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Citations

Sep 16, 2017·Journal of Food and Drug Analysis·Jia-Ching WuMin-Hsiung Pan
Nov 20, 2016·The Laryngoscope·Tanaya WalimbeM Preeti Sivasankar
Jul 25, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Arpita BasuJeffrey L Ebersole
Nov 11, 2019·BMC Oral Health·Takuya NiiYoichiro Miyake
Jul 1, 2020·Frontiers in Bioengineering and Biotechnology·Ziba NajmiLia Rimondini
Apr 12, 2019·Archives of Oral Biology·Juliano Milanezi de AlmeidaEdilson Ervolino
Apr 12, 2019·Archives of Oral Biology·Yuan LiRuijie Huang

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