Green Tea Polyphenols Attenuated Glutamate Excitotoxicity via Antioxidative and Antiapoptotic Pathway in the Primary Cultured Cortical Neurons

Oxidative Medicine and Cellular Longevity
Lin CongXiao-Yan Qin

Abstract

Green tea polyphenols are a natural product which has antioxidative and antiapoptotic effects. It has been shown that glutamate excitotoxicity induced oxidative stress is linked to neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease. In this study we explored the neuroprotective effect of green teen polyphenols against glutamate excitotoxicity in the primary cultured cortical neurons. We found that green tea polyphenols protected against glutamate induced neurotoxicity in the cortical neurons as measured by MTT and TUNEL assays. Green tea polyphenols were then showed to inhibit the glutamate induced ROS release and SOD activity reduction in the neurons. Furthermore, our results demonstrated that green tea polyphenols restored the dysfunction of mitochondrial pro- or antiapoptotic proteins Bax, Bcl-2, and caspase-3 caused by glutamate. Interestingly, the neuroprotective effect of green tea polyphenols was abrogated when the neurons were incubated with siBcl-2. Taken together, these results demonstrated that green tea polyphenols protected against glutamate excitotoxicity through antioxidative and antiapoptotic pathways.

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Citations

May 20, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kyung Hoan ImTae Soo Lee
Jun 15, 2016·Pain Management·Kathleen Holton
Feb 23, 2018·Current Drug Discovery Technologies·Tahereh FarkhondehSaeed Samarghandian
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Nov 20, 2018·Journal of Agricultural and Food Chemistry·Xiu-Hua MengYing-Jun Zhang
Sep 3, 2021·Oxidative Medicine and Cellular Longevity·Natália Cruz-MartinsWilliam C Cho

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Methods Mentioned

BETA
Assay
fluorescence spectroscopy

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