Green Tea Polyphenols Reduce Gastric Epithelial Cell Proliferation and Apoptosis Stimulated by Helicobacter pylori Infection

Journal of Clinical Biochemistry and Nutrition
Yuichi AkaiY Arakawa

Abstract

Recently the finding of gastric cancer in Helicobacter pylori (H. pylori)-infected mouse models was reported. Studies of humans and animal models have shown that H. pylori infection stimulates gastric epithelial cell proliferation and apoptosis. Polyphenols contained in green tea and related compounds were reported to have a variety anti-tumor effects and bactericidal properties. We studied the effect of green tea polyphenols on gastric cell proliferation and apoptosis in an H. pylori-infected mouse model. This model was prepared by inoculating Balb/c mice with 10(8) cfu of H. pylori (NCTC 11637 strain) by gavage. Beginning 18 weeks after inoculation, 0.5% polyphenols were given in drinking water every day for 2 weeks. Mice were sacrificed 1 h after bromodeoxyuridine (BrdU) was given i.p. for preparation of paraffin-embedded specimens. Cell proliferation and apoptosis were examined by the avidin-biotin complex method using anti-BrdU antibody and the TUNEL method, respectively. H. pylori infection resulted in increased BrdU-labeled cells in both the antrum and the bodies. Administration of polyphenols suppressed this increased proliferation. H. pylori infection increased apoptotic cells in both the antrum and the corpus in compa...Continue Reading

References

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Citations

Mar 25, 2009·Journal of Clinical Biochemistry and Nutrition·Noriko NakajimaMitsuhiko Moriyama
Aug 21, 2013·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Antonietta RizzoMaria Rosaria Iovene
Nov 26, 2010·International Immunopharmacology·Rossella PaolilloAntonietta Rizzo
May 22, 2010·Journal of Agricultural and Food Chemistry·Edgar PasteneGuillermo Figueroa

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Methods Mentioned

BETA
light microscopy

Software Mentioned

pro
Image
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