Green tissue-specific co-expression of chitinase and oxalate oxidase 4 genes in rice for enhanced resistance against sheath blight

Planta
Subhasis KarmakarKarabi Datta

Abstract

Green tissue-specific simultaneous overexpression of two defense-related genes ( OsCHI11 & OsOXO4 ) in rice leads to significant resistance against sheath blight pathogen ( R. solani ) without distressing any agronomically important traits. Overexpressing two defense-related genes (OsOXO4 and OsCHI11) cloned from rice is effective at enhancing resistance against sheath blight caused by Rhizoctonia solani. These genes were expressed under the control of two different green tissue-specific promoters, viz. maize phosphoenolpyruvate carboxylase gene promoter, PEPC, and rice cis-acting 544-bp DNA element, immediately upstream of the D54O translational start site, P D54O-544 . Putative T0 transgenic rice plants were screened by PCR and integration of genes was confirmed by Southern hybridization of progeny (T1) rice plants. Successful expression of OsOXO4 and OsCHI11 in all tested plants was confirmed. Expression of PR genes increased significantly following pathogen infection in overexpressing transgenic plants. Following infection, transgenic plants exhibited elevated hydrogen peroxide levels, significant changes in activity of ROS scavenging enzymes and reduced membrane damage when compared to their wild-type counterpart. In a Rhi...Continue Reading

References

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Citations

Nov 8, 2017·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Xiang LiYuan Li
Dec 26, 2018·Plant Biotechnology Journal·Banpu RuanZhenyu Gao
Nov 13, 2019·Functional & Integrative Genomics·Hong-Le WangHui Xie
May 1, 2019·Molecular Plant-microbe Interactions : MPMI·Zhiqin LiuShuilin He
Dec 8, 2019·Plant Biotechnology Journal·Kutubuddin A MollaKarabi Datta
Feb 4, 2019·World Journal of Microbiology & Biotechnology·Guangzheng SunQing Ma
Jul 13, 2018·International Journal of Molecular Sciences·Mande XueXinwu Pei

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