Green Transfection: Cationic Lipid Nanocarrier System Derivatized from Vegetable Fat, Palmstearin Enhances Nucleic Acid Transfections

ACS Omega
Priya DharmalingamSrujan Kumar Marepally

Abstract

Cationic lipid-guided nucleic acid delivery holds great promise in gene therapy and genome-editing applications for treating genetic diseases. However, the major challenge lies in achieving therapeutically relevant efficiencies. Prior findings, including our own, demonstrated that asymmetry in the hydrophobic core of cationic lipids imparted superior transfection efficiencies. To this end, we have developed a lipid nanocarrier system with an asymmetric hydrophobic core (PS-Lips) derived from a mixture of fatty acids of food-grade palmstearin and compared its efficiency with symmetric palmitic acid-based nanocarrier system (P-Lip). PS-Lips exhibited superior transfection efficiencies with both plasmid DNA (pDNA) and mRNA in multiple cultured cells than the control P-Lip. More importantly, PS-Lips exhibited 2-fold superior transfections with linear nucleic acid, green fluorescent protein (GFP) mRNA in hematopoietic cells, when compared with the commercial control lipofectamine RNAiMAX. PS-Lips was also found to be effective in delivering genome-editing tools (CRISPR/Cas9, sgRNA encoded pDNA with a reporter GFP construct) than P-Lip in HEK-293 cells. In the present study, we report that cationic liposomes derivatized from natural ...Continue Reading

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Citations

Feb 9, 2019·Biomaterials Science·Joon Eoh, Luo Gu
Feb 25, 2020·Methods and Applications in Fluorescence·Lucia BoeriManuela Teresa Raimondi
May 5, 2018·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Bin LiYizhou Dong
Jun 15, 2018·Biochimica Et Biophysica Acta. Biomembranes·Vignesh K RangasamiSrujan Marepally
Nov 24, 2019·Advanced Drug Delivery Reviews·Cong-Fei XuJun Wang
Aug 8, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Santhosh Chandar MaddilaSrujan Marepally

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Methods Mentioned

BETA
transfection
dynamic light scattering
transfections
FRET
nuclear
electrophoresis
FACS
Fluorescence

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