Griffithsin binds to the glycosylated proteins (E and prM) of Japanese encephalitis virus and inhibit its infection.

Virus Research
Hassan Z A IshagXiang Mao

Abstract

Griffithsin (GRFT) is a broad-spectrum antiviral protein against several glycosylated viruses. In our previous publication, we have shown that GRFT exerted antiviral activity against Japanese encephalitis virus (JEV) infection. Herein, we further elucidated the mechanism by which GRFT inhibits JEV infection in BHK-21 cells. In vitro experiments using Pull-down assay and Co-immunoprecipitation (CO-IP) assay showed that GRFT binds to the JEV glycosylated viral proteins, specifically the enveloped (E) and premature (prM) glycoproteins. The binding of GRFT to the JEV was competitively inhibited by increasing concentrations of mannose; in turns abolished anti-JEV activity of GRFT. We suggested that, the binding of GRFT to the glycosylated viral proteins may contribute to its anti-JEV activity. Collectively, our data indicated a possible mechanism by which GRFT exerted its anti-JEV activity. This observation suggests GRFT's potentials in the development of therapeutics against JEV or other flavivirus infection.

Citations

Jan 1, 2018·Journal of Applied Phycology·Ram Sarup Singh, Amandeep Kaur Walia
Jun 2, 2018·BioMed Research International·Milena Mazalovska, J Calvin Kouokam
Oct 27, 2016·Viruses·Sabrina Lusvarghi, Carole A Bewley
Jul 12, 2018·Expert Opinion on Biological Therapy·Luisa Barzon, Giorgio Palù
May 5, 2017·Pharmaceuticals·So Young KimRobert J Linhardt
Dec 1, 2020·Frontiers in Cellular and Infection Microbiology·Punya Shrivastava-RanjanChristina F Spiropoulou
Aug 29, 2021·Pharmaceuticals·Nour Mammari On Behalf Of The Oemonom

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Methods Mentioned

BETA
glycosylation
PCR
Pull-down
Co-immunoprecipitation
Co-IP
Pulldown

Software Mentioned

SPSS

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