GroEL is an immunodominant surface-exposed antigen of Rickettsia typhi.

PloS One
Jessica RauchAnke Osterloh

Abstract

Rickettsioses are neglected and emerging potentially fatal febrile diseases that are caused by obligate intracellular bacteria, rickettsiae. Rickettsia (R.) typhi and R. prowazekii constitute the typhus group (TG) of rickettsiae and are the causative agents of endemic and epidemic typhus, respectively. We recently generated a monoclonal antibody (BNI52) against R. typhi. Characterization of BNI52 revealed that it specifically recognizes TG rickettsiae but not the members of the spotted fever group (SFG) rickettsiae. We further show that BNI52 binds to protein fragments of ±30 kDa that are exposed on the bacterial surface and also present in the periplasmic space. These protein fragments apparently derive from the cytosolic GroEL protein of R. typhi and are also recognized by antibodies in the sera from patients and infected mice. Furthermore, BNI52 opsonizes the bacteria for the uptake by antigen presenting cells (APC), indicating a contribution of GroEL-specific antibodies to protective immunity. Finally, it is interesting that the GroEL protein belongs to 32 proteins that are differentially downregulated by R. typhi after passage through immunodeficient BALB/c CB17 SCID mice. This could be a hint that the rickettsia GroEL pro...Continue Reading

References

Feb 10, 1983·Nature·G C BosmaM J Bosma
Feb 1, 1994·European Journal of Epidemiology·D RaoultO Ramousse
Mar 1, 1993·Microbiological Reviews·A P Pugsley
Nov 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·H T DupontD Raoult
Apr 1, 1997·Protein Science : a Publication of the Protein Society·W A Fenton, A L Horwich
Jan 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·E S BochkarevaA S Girshovich
Apr 3, 1999·FEMS Microbiology Letters·J JyotA Ghosh
Jun 17, 1999·Lancet·A SteinD Raoult
May 19, 2000·Antimicrobial Agents and Chemotherapy·D TurcinovB Herendić
Jul 7, 2000·Clinical and Diagnostic Laboratory Immunology·B La ScolaD Raoult
Jul 19, 2000·The Journal of Experimental Medicine·C FratazziH G Remold
Apr 27, 2001·Lancet·L I Lutwick
Sep 6, 2002·Molecular Immunology·Abu Salim Mustafa
Mar 13, 2003·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·F BacellarA.R. Filipe
May 10, 2003·Emerging Infectious Diseases·Philippe ParolaDidier Raoult
Apr 15, 2004·Infectious Disease Clinics of North America·Didier RaoultJ Stephen Dumler
Aug 20, 2004·Journal of Bacteriology·Michael P McLeodGeorge M Weinstock
Nov 17, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Mogens JenseniusDidier Raoult
Dec 15, 2005·The Journal of Biological Chemistry·Louise BaarsJan-Willem de Gier
Feb 17, 2006·Annals of the New York Academy of Sciences·Patricia RenestoDidier Raoult
Feb 24, 2006·Emerging Infectious Diseases·Simaly PhongmanyPaul N Newton
Jun 13, 2006·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M Bernabeu-WittelJ Pachón
Jun 8, 2007·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M KoliouA Gikas
Nov 21, 2007·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Piyush Kumar PaliwalIlavazhagan Govindaswamy
Mar 26, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kristoffer WeberBoris Fehse
Jul 8, 2008·Nature Immunology·Annett HalleDouglas T Golenbock
Aug 14, 2008·Journal of Vector Ecology : Journal of the Society for Vector Ecology·Will K ReevesAmanda D Loftis

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Software Mentioned

Andromeda
Perseus
MaxQuant
GraphPad
Glycopp
MPSite
GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Clinical Microbiology
Jung-Hee LeeSeung-Hyun Lee
Nihon rinsho. Japanese journal of clinical medicine
Y YoshidaT Katayama
British Medical Journal
Frontiers in Microbiology
Tsuneo Uchiyama
© 2021 Meta ULC. All rights reserved