PMID: 9557645Apr 29, 1998Paper

Grossly defective nef gene sequences in a human immunodeficiency virus type 1-seropositive long-term nonprogressor

Journal of Virology
R SalviA Benedetto

Abstract

We have been investigating a long-term nonprogressor who was found to be human immunodeficiency virus type 1 (HIV-1) seropositive in 1985 and has survived with stable CD4+ T-cell counts (>1,000 CD4 cells/microl) without any AIDS-related illness. We have previously reported that repeated attempts to measure HIV-1 RNA in the peripheral mononuclear cells obtained from this subject have invariably failed. In the present study, we have analyzed the molecular nature of the HIV-1 quasispecies infecting this patient by PCR amplification of two proviral regions, the 5' long terminal repeat (5'LTR)/gag leader and the nef gene, directly from fresh uncultured peripheral mononuclear cells, followed by length polymorphism analysis (with 1994, 1995, and 1996 samples) and sequencing (with a 1996 sample). Only proviral forms with nef deletions were revealed by length polymorphism analysis in samples from all three time points. Sequence analysis of the nef gene from the 1996 sample confirmed the presence of similar proviral quasispecies characterized by the presence of several deletions located in the nef-alone and the nef/U3 overlapping regions. Length polymorphism analysis of the 5'LTR/gag leader region suggested the existence of two major qua...Continue Reading

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Citations

May 16, 2000·The Journal of Biological Chemistry·G B CohenD Baltimore
Aug 21, 2001·Journal of Acquired Immune Deficiency Syndromes : JAIDS·L A Cramer, J A Frelinger
Sep 29, 2004·Journal of Virology·Ming-Bo HuangVincent C Bond
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May 19, 2009·BioFactors·Maria Giovanna QuarantaMarina Viora
Dec 17, 2009·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Vanessa Rivera-AmillAnil Kumar
Sep 17, 2010·PloS One·Alicia ArnottUNKNOWN Pulse Study Team
Apr 13, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Catherine Yi CaiYong-Jiu Jin
Apr 29, 2015·PloS One·Xiaoyu LuoJohnny J He
Nov 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·J SidneyA Sette
Dec 16, 2000·AIDS Research and Human Retroviruses·R GeffinA S Baur
Jul 3, 2002·Proceedings of the National Academy of Sciences of the United States of America·Mohan SomasundaranMario Stevenson
Sep 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Allison OlszewskiGregory A Weiss
Feb 17, 2010·AIDS Research and Human Retroviruses·Syed A AliVincent C Bond
Nov 10, 2009·AIDS Research and Human Retroviruses·D B TzitzivacosM A Papathanasopoulos

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