PMID: 7914103Apr 1, 1993

Group A streptococcal polysaccharide--an inhibitor of the functional activity of T-suppressors induced by Con A in a culture of mononuclear cells of human peripheral blood

Biulleten' eksperimental'noĭ biologii i meditsiny
E V GnezitskaiaV Iu Sanina

Abstract

It was found that group A streptococcal polysaccharide (A-PS) had no mitogenic effect on the intact human blood mononuclear cells' culture (MNC) and on its proliferation stimulated with ConA. Using a double staining technique for simultaneously determining cell surface phenotype and degree of cell activation by it's ability to include the tetrazolium dye MTT (3-[4,5-dimethylthiazol-2-yl] -2,5-diphenyl tetrazolium bromide) it was established that A-PS decreased the percent of activated (MTT+) cells in the subpopulation of CD8+ and increased the percent of MTT+ cells among CD4+ lymphocytes in the intact MNC. In the MNC stimulated with ConA A-PS caused only one of these effects: it decreased the percent of MTT+ cells in the subpopulation of CD8+ lymphocytes. The correlation was established between the results obtained with MTT-technique and cell transfer test, because the MNC preincubated with ConA and A-PS lost the ability to suppresses MNC proliferation stimulated with PHA. The data obtained supported the assumption that A-PS as a carrier of determinants common with thymus epidermal antigens (factors) may act as it's functional analogue and thus promote the development of autoimmune process during the rheumatic fever.

Citations

Feb 1, 1995·Biulleten' eksperimental'noĭ biologii i meditsiny·E A BazanovaN N Ignatenko
Dec 22, 1999·Biulleten' eksperimental'noĭ biologii i meditsiny·E A BazanovaV G Nesterenko

Related Concepts

Parent of thiazolyl blue
streptococcal polysaccharide group A
Cell Division Phases
Concanavalin A
Organic Pigments
Nongranular Leukocyte
Polysaccharides, Bacterial
Bacteria, Flesh-Eating
Tetrazolium Salts
Thiazoles

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