Group A streptococcus adheres to pharyngeal epithelial cells with salivary proline-rich proteins via GrpE chaperone protein.

The Journal of Biological Chemistry
Jumpei MurakamiS Kawabata

Abstract

Group A Streptococcus pyogenes (GAS) is an important human pathogen that frequently causes pharyngitis. GAS organisms can adhere to and invade pharyngeal epithelial cells, which are overlaid by salivary components. However, the role of salivary components in GAS adhesion to pharyngeal cells has not been reported precisely. We collected human saliva and purified various salivary components, including proline-rich protein (PRP), statherin, and amylase, and performed invasion assays. The GAS-HEp-2 association ratio (invasion/adhesion ratio) and invasion ratio of GAS were increased significantly with whole human saliva and PRP, while the anti-PRP antibody inhibited the latter. GAS strain NY-5, which lacks M and F proteins on the cell surface, was promoted to cohere with HEp-2 cells by whole human saliva and PRP. The 28-kDa protein of GAS bound to PRP and was identified as GrpE, a chaperone protein, whereas the N-terminal of GrpE was found to bind to PRP. A GrpE-deficient mutant of GAS strain B514Sm, TR-45, exhibited a reduced ability to adhere to and invade HEp-2 cells. Microscopic observations showed the GrpE was mainly expressed on the surface of the cell division site of GAS. Furthermore, GrpE-deficient mutants of GAS and Strept...Continue Reading

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Citations

May 28, 2014·Canadian Journal of Microbiology·Tatsuma AsohShin-ichi Yoshida
Dec 3, 2014·Immunology Letters·Massimo Costalonga, Mark C Herzberg
Dec 24, 2014·Journal of Proteomics·Fábio TrindadeRui Vitorino
Apr 5, 2014·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Lorenzo DragoRoberto Mattina
Jan 15, 2015·Applied Microbiology and Biotechnology·João Pinto da CostaRui Vitorino
Jun 18, 2016·FEBS Letters·Stephan BrouwerMark J Walker
Jul 3, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·David M P De OliveiraMartina L Sanderson-Smith
Feb 16, 2019·Microbiology Spectrum·Vijay Pancholi

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