PMID: 2094423Jan 1, 1990Paper

Group separation of free and conjugated bile acids by pre-packed anion-exchange cartridges

Journal of Pharmaceutical and Biomedical Analysis
S Scalia

Abstract

A rapid and simple procedure for the group separation of the major free and conjugated bile acids of man is described. After initial extraction with Bond Elut C18 cartridges, the analytes are fractionated into the unconjugated, glycine- and taurine-conjugated forms using disposable Bond Elut SAX columns and methanol-acetate buffer eluents. The method is found to be accurate and reproducible and to afford complete resolution between fractions. Free and conjugated bile acids present in human bile and gastric juice are assayed by high-performance liquid chromatography, after extraction and group separation according to the described procedure.

References

Jul 1, 1978·Clinica Chimica Acta; International Journal of Clinical Chemistry·J GotoT Nambara
Nov 1, 1985·Gastroenterology·A B StefaniwskyG Salen
Jan 7, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·K D Setchell, A Matsui
Feb 26, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·A T Ruben, G P van Berge-Henegouwen
Oct 8, 1981·Clinica Chimica Acta; International Journal of Clinical Chemistry·A V GrecoS Passi
Jan 1, 1988·Journal of Pharmaceutical and Biomedical Analysis·S ScaliaM Guarneri

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Citations

Jan 1, 1997·Biomedical Chromatography : BMC·R GattiV Cavrini
Jan 31, 1994·Clinica Chimica Acta; International Journal of Clinical Chemistry·S Scalia, P Pazzi
Feb 1, 1995·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·P SimoniA Roda
Feb 26, 2010·Isotopes in Environmental and Health Studies·Frans StellaardFolkert Kuipers
Dec 17, 2009·Journal of Lipid Research·William J Griffiths, Jan Sjövall
Aug 21, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Gemma BrufauElizabeth J Murphy
Dec 1, 1995·Scandinavian Journal of Gastroenterology·O VillangerM G Raeder
Dec 1, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·A RodaM Baraldini
Mar 24, 1995·Journal of Chromatography. B, Biomedical Applications·A RodaM Baraldini
Sep 15, 1995·Journal of Chromatography. B, Biomedical Applications·S Scalia

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