Group-Specific 16S rRNA-Targeted Oligonucleotide Probes To Identify Thermophilic Bacteria in Marine Hydrothermal Vents.

Applied and Environmental Microbiology
H J HarmsenChristian Jeanthon

Abstract

Four 16S rRNA-targeted oligonucleotide probes were designed for the detection of thermophilic members of the domain Bacteria known to thrive in marine hydrothermal systems. We developed and characterized probes encompassing most of the thermophilic members of the genus Bacillus, most species of the genus Thermus, the genera Thermotoga and Thermosipho, and the Aquificales order. The temperature of dissociation of each probe was determined. Probe specificities to the target groups were demonstrated by whole-cell and dot blot hybridization against a collection of target and nontarget rRNAs. Whole-cell hybridizations with the specific probes were performed on cells extracted from hydrothermal vent chimneys. One of the samples contained cells that hybridized to the probe specific to genera Thermotoga and Thermosipho. No positive signals could be detected in the samples tested with the probes whose specificities encompassed either the genus Thermus or the thermophilic members of the genus Bacillus. However, when simultaneous hybridizations with the probe specific to the order Aquificales and a probe specific to the domain Bacteria (R. I. Amann, B. Binder, R. J. Olson, S. W. Chisholm, R. Devereux, and D. A. Stahl, Appl. Environ. Micro...Continue Reading

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Citations

Jun 6, 2003·Applied and Environmental Microbiology·Matthew O SchrenkJohn A Baross
Jun 17, 2003·International Journal of Systematic and Evolutionary Microbiology·Satoshi NakagawaYoshihiko Sako
May 17, 2017·Microorganisms·Annarita PoliBarbara Nicolaus
May 28, 2013·Extremophiles : Life Under Extreme Conditions·Kerstin SahmGarabed Antranikian
May 29, 2004·Extremophiles : Life Under Extreme Conditions·Antje Rusch, Jan P Amend
Jan 19, 2008·Environmental Microbiology·Antoine PagéAnna-Louise Reysenbach
Jul 27, 2001·Applied and Environmental Microbiology·K TakaiK Horikoshi
Aug 30, 2001·Applied and Environmental Microbiology·V T MarteinssonJ K Kristjánsson
Nov 27, 2002·Applied and Environmental Microbiology·Mircea PodarMitchell L Sogin
Dec 9, 2003·Applied and Environmental Microbiology·Tatsunori Nakagawa, Manabu Fukui

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