Growing and Genetically Manipulating Human Umbilical Cord Blood-Derived Erythroid Progenitor (HUDEP) Cell Lines

Methods in Molecular Biology
Divya S Vinjamur, Daniel E Bauer

Abstract

The recently established human umbilical cord blood-derived erythroid progenitor (HUDEP) cell lines have equipped red blood cell researchers with valuable in vitro models of erythroid development. Of the three established HUDEP cell lines, HUDEP-2 cells express predominantly adult β-globin and most closely resemble adult erythroid cells. This chapter describes culture protocols for the maintenance and erythroid differentiation of HUDEP-2 cells. Methods to genetically manipulate HUDEP-2 cells using a CRISPR/Cas9 nuclease-based approach are also discussed.

Citations

Jun 14, 2020·Journal of Translational Medicine·Yu Jin ParkHyun Ok Kim
Apr 17, 2021·Nature Genetics·Divya S VinjamurDaniel E Bauer
Apr 23, 2021·American Journal of Human Genetics·Yao HuUNKNOWN NHLBI Trans-Omics for Precision Medicine (TOPMed) Consortium
Nov 25, 2021·Science Advances·Richard KingRami Khoriaty

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