Growing Murine Bone Marrow-Derived Macrophages

Methods in Molecular Biology
Anaïs AssouvieGermain Rousselet

Abstract

Bone marrow-derived macrophages (BMDM) are primary macrophages obtained by in vitro differentiation of bone marrow cells in the presence of macrophage colony-stimulating factor (M-CSF or CSF1). They are easy to obtain in high yields, can be stored by freezing, and can be obtained from genetically modified mice strains. They are therefore widely used as prototypical macrophages for in vitro studies. In this chapter, we present the method for obtaining BMDMs and freezing them.

Citations

Dec 7, 2018·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Shenhai GongPeng Chen
Aug 9, 2020·Frontiers in Immunology·Leah R ZuroffMaya Koronyo-Hamaoui
Mar 23, 2021·Journal of the American Heart Association·Min ZhouWeiguo Fu
Apr 16, 2021·Life Science Alliance·Rachel E HeapMatthias Trost

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