Growth and development of Gnathostoma spinigerum early third-stage larvae in vitro

Journal of Helminthology
Wanchai MaleewongN Morakote

Abstract

Early third-stage larvae of Gnathostoma spinigerum were cultured in RPMI-1640 supplemented with 25mM NaHCO3,100 units/ml penicillin G, 100 microg/ml of streptomycin, 5 microg/ml of amphotericin B and 10% foetal calf serum at 37 degrees C under 5% CO2 in air for 60 days. After 3 days of cultivation, the larvae moulted. Body sizes increased from 0.49 +/- 0.09 x 0.07 +/- 0.01 mm in length and width to 4.08 +/- 0.48 x 0.32 +/- 0.04 mm after 60 days of cultivation. The maximum body length and width of these larvae were 4.94 mm and 0. 35 mm, respectively. The survival rate (67.5 %) of the worms was observed at the end of cultivation. The addition of foetal calf serum was found to be essential for growth and development.

References

Mar 1, 1977·Journal of the Neurological Sciences·P BoongirdA Vejjajiva
Mar 1, 1992·Journal of Helminthology·K AndoY Chinzei
Jan 1, 1992·The American Journal of Tropical Medicine and Hygiene·W MaleewongN Morakote
Jun 1, 1991·International Journal for Parasitology·P TapchaisriP Setasuban
Sep 1, 1988·Parasitology Today·N Jaroonvesama

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