PMID: 12757880May 22, 2003Paper

Growth and differentiation of rat bone marrow stromal cells: does 5-azacytidine trigger their cardiomyogenic differentiation?

Cardiovascular Research
Yu LiuChengjun Hu

Abstract

The potential use of bone marrow stromal cells (MSCs) as a cellular therapy for chronic cardiac diseases relies on the ability of the cell to replicate extensively in vitro and to give rise to myogenic cells that can replace the damaged cardiomyocytes. For this reason the present study investigated the replication lifespan and chemical-induced cardiomyogenic differentiation of rat MSCs in vitro. The primary and the successively passaged Wistar rat MSCs were exposed to different concentrations (3, 5 and 10 microM) of 5-azacytidine using different methods (single- or repeat-treatment). The growth properties and the fate of the cells were compared to their untreated counterparts by cell counting, immunocytochemistry and Western analysis. When seeded at a density of 2845 cells/cm(2) and cultured under common conditions, rat MSCs could be expanded up to 21.94 cell doublings in 30 days of successive subcultures. This was accompanied by a gradual loss of their replication ability with passages. When treated with 5-azacytidine for 24 h at day 3 of primary culture and the first subculture, the growth properties of the MSCs were not obviously affected. Neither the spontaneously beating cells nor the formation of myotubes were found in th...Continue Reading

Citations

Feb 9, 2008·Archives of Dermatological Research·Paolo SbanoRiccardo Saccardi
Jun 19, 2009·Journal of Artificial Organs : the Official Journal of the Japanese Society for Artificial Organs·Azizi MiskonTetsuji Yamaoka
Jun 4, 2011·Cardiovascular Drugs and Therapy·Drew KuraitisErik J Suuronen
Oct 10, 2007·Veterinary Research Communications·M FerrariA Lanfranchi
Feb 5, 2004·The Annals of Thoracic Surgery·Yong-Fu XiaoJames P Morgan
Nov 12, 2010·Stem Cells and Development·Ana-Maria Rosca, Alexandrina Burlacu
Mar 24, 2005·Journal of Cellular and Molecular Medicine·Anke M SmitsPieter A Doevendans
Nov 8, 2008·Circulation Research·Hans ReineckeMichael A Laflamme
Jan 10, 2012·Journal of Negative Results in Biomedicine·Wan Kamarul Zaman Wan SafwaniKien Hui Chua
Jan 15, 2009·Folia Histochemica Et Cytobiologica·Anna BurdzińskaLeszek Paczek
Dec 3, 2013·Canadian Journal of Physiology and Pharmacology·Sreejit ParameswaranRajendra K Sharma
Sep 17, 2015·Artificial Cells, Nanomedicine, and Biotechnology·Xiao-Ling ZhaoYan-Hua Dong
Mar 1, 2012·Differentiation; Research in Biological Diversity·Faizal Z Asumda, P Bryant Chase
Jan 19, 2008·International Journal of Cardiology·Wen-Chi C LeeKacey G Marra
Jun 17, 2004·British Journal of Haematology·William B StrawnCarlos M Ferrario
Jun 11, 2009·Journal of Cellular and Molecular Medicine·Hua LiuTong Cao
Dec 14, 2011·Journal of Tissue Engineering and Regenerative Medicine·Isabel MoscosoNieves Domenech
Jan 5, 2011·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·D Adam YoungKaren L Christman
Jan 15, 2010·Journal of Cellular and Molecular Medicine·Yu Suk ChoiRodney J Dilley
Jan 3, 2006·Experimental Cell Research·Bénédicte Prunet-MarcassusLouis Casteilla
Sep 7, 2005·Biochemical and Biophysical Research Communications·Fa-Bao ZhangPing-Jin Gao
Jul 5, 2008·Stem Cells·Peter J PsaltisStan Gronthos
May 13, 2006·Genomics, Proteomics & Bioinformatics·Neng-Sheng YeGuo-An Luo
Apr 17, 2008·Biochemical and Biophysical Research Communications·Kenichi YamaharaKen Suzuki

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.