PMID: 43891Aug 1, 1979

Growth and differentiation of Trypanosoma cruzi cultivated with a Triatoma infestans embryo cell line

The Journal of protozoology
D E Lanar


Trypanosoma cruzi strain Peru was cultivated in the presence of an established cell line of Triatoma infestans embryo cells (TI- 32). Bloodstream trypomastigotes differentiated into amastigote-like cells (first differentiation phase) which multiplied to form large clusters of cells. Because of their clustering nature, a new term, "staphylomastigotes," has been proposed for this stage. After 10 days of cultivation, 90% of the staphylomastigotes underwent differentiation (2nd differentiation phase) to trypomastigotes (approximately 98%) or epimastigotes (approximately 2%). Bloodstream trypomastigotes cultivated without TI-32 cells underwent the first, but not the 2nd differentiation phase, although occasional epimastogotes were seen (less than 1%). The evidence presented suggests that TI-32 cells produce a labile factor(s) important not only for initiation of the 2nd differentiation phase but also for maintaining the parasites in the trypomastigote stage. The pH of the culture medium was not the initiating factor for the 2nd differentiation phase. Infectivity studies indicated that staphylomastigotes were as infective as blood stream trypomastigotes, but that metacyclic trypomastigotes isolated from culture after the 2nd differen...Continue Reading


Jan 1, 1982·Zeitschrift Für Parasitenkunde·D G Dusanic, R J Boisvenue
Apr 1, 1984·Molecular and Biochemical Parasitology·D E Lanar, J E Manning
Sep 1, 1985·Molecular and Biochemical Parasitology·E A DragonJ E Manning
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Apr 1, 1976·Experimental Parasitology·K P Wagner
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Related Concepts

Embryo Loss
Entire Embryo
Factor V
Transcription Initiation
Cell Differentiation Process
Embryonic Structures, Nonmammalian
Blood Circulation
Trypanosoma cruzi
Amastigote form of protozoa

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