Growth and EGFR regulation in breast cancer cells by vitamin D and retinoid compounds

Breast Cancer Research and Treatment
Kenneth R McGaffinS A Chrysogelos

Abstract

The effect of 1,25-dihydroxyvitamin D(3), analog C (1,25-(OH)(2)-16-en-23-yn-26,27-F(6)-vitamin D(3)), 9-cis retinoic acid, and all-trans retinoic acid on the growth and expression of EGFR in MCF7, T47D, BT474, and BT549 breast cancer cells was examined. Significant growth inhibition was noted in MCF7, T47D, and BT474 cells by 8 days of treatment, while BT549 cells showed none. MCF7, T47D, and BT549 cells treated with 1,25-dihydroxyvitamin D(3) demonstrated a 50% decrease in EGFR mRNA within 2 h which was sustained to 72 h, while BT474 cells demonstrated a 200-500% increase. EGFR protein levels correlated with these mRNA changes in BT474 and BT549 cells. Measurement of mRNA stability in vitamin D treated BT474 cells indicated that there was no change in EGFR mRNA half-life. Transfection of an EGFR promoter containing reporter plasmid demonstrated vitamin D induced changes in reporter gene activity that paralleled the changes observed in EGFR mRNA and protein. Electrophoretic mobility shift assays using a putative vitamin D response element within this region of the EGFR promoter demonstrated specific VDR binding. These results indicate that the vitamin D effect on EGFR expression in breast cancer cells has a transcriptional com...Continue Reading

References

Nov 1, 1977·Journal of the National Cancer Institute·W J BlotB J Stone
Jan 1, 1991·Annual Review of Nutrition·J W Pike
Oct 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·M Koga, R L Sutherland
Mar 23, 1991·Lancet·M BowerR C Combes
Apr 15, 1991·Biochemical and Biophysical Research Communications·P Y DesprezS Saez
Sep 1, 1991·Molecular and Cellular Endocrinology·I Nemere, A W Norman
Dec 1, 1990·International Journal of Epidemiology·E D GorhamC F Garland
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M B DemayH M Kronenberg
Apr 20, 1990·Cell·A Ullrich, J Schlessinger
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A J ClarkI Pastan
Jan 1, 1994·Breast Cancer Research and Treatment·R R BurasS R Evans
Jan 1, 1993·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·L JardinesM Greene
Jan 1, 1994·Breast Cancer Research and Treatment·T Rajkumar, W J Gullick
Jan 1, 1994·Breast Cancer Research and Treatment·J Baselga, J Mendelsohn
Jan 1, 1994·Breast Cancer Research and Treatment·S A Chrysogelos, R B Dickson
May 1, 1994·Clinical & Experimental Metastasis·C M HansenL Binderup
Sep 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·B GrosseM Lieberherr
Jul 22, 1993·The New England Journal of Medicine·D J HunterW C Willett
Jun 1, 1993·Journal of Cellular Biochemistry·A M SorensenD T Baran
Dec 15, 1995·Cell·D J MangelsdorfR M Evans
May 1, 1997·Cellular Signalling·A D Laird, D Shalloway
Oct 8, 1999·Public Health Nutrition·E C JanowskyB S Hulka
Feb 11, 2000·Annals of the New York Academy of Sciences·C F GarlandE D Gorham
Aug 6, 2000·Circulation Research·D J MantellA E Canfield
Dec 18, 2001·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·P BortmanM M Brentani
Mar 26, 2003·The Journal of Steroid Biochemistry and Molecular Biology·JoEllen WelshCarmen J Narvaez

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Citations

Mar 19, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Nicolas DuhemVéronique Préat
Aug 5, 2009·Critical Reviews in Clinical Laboratory Sciences·Elzbieta Gocek, George P Studzinski
Jun 28, 2011·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Luisa SalvatoriElisa Petrangeli
Jan 19, 2006·The Journal of Investigative Dermatology·Danielle Paixão-CavalcanteDenise V Tambourgi
May 14, 2009·International Journal of Cancer. Journal International Du Cancer·Takuya MohriTsuyoshi Yokoi
Apr 23, 2009·Journal of Cellular and Molecular Medicine·Darja ObradovicTheo Rein
Jul 19, 2013·International Journal of Cosmetic Science·K v q Lu'o'ng, L T H Nguyễn
Aug 8, 2006·Biochimica Et Biophysica Acta·Sinto SebastianAngelo Paradiso
Jul 9, 2005·Trends in Endocrinology and Metabolism : TEM·Ricardo BolandAna Russo De Boland
Aug 4, 2005·The Journal of Steroid Biochemistry and Molecular Biology·LaMonica V StewartNancy L Weigel
Jun 9, 2012·European Journal of Medicinal Chemistry·Marwa N Abu-AishehMohammad S Mubarak
May 1, 2017·Oncotarget·Vivien HichertJulia Stingl
May 20, 2019·Journal of Cellular Biochemistry·Xiaolong LiangZheng Xiang

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