Growth and magnetosome formation by microaerophilic Magnetospirillum strains in an oxygen-controlled fermentor

Applied Microbiology and Biotechnology
U Heyen, D Schüler

Abstract

Media and growth conditions were optimized for the microaerobic cultivation of Magnetospirillum gryphiswaldense in flasks and in a fermentor, resulting in significantly increased cell and magnetosome yields, compared with earlier studies. A reliable method was established for the automatic control of low dissolved oxygen tensions (pO(2)) in the fermentor (oxystat). Growth and magnetosome formation by M. gryphiswaldense, M. magnetotacticum and Magnetospirillum sp. AMB-1 were studied at various oxygen concentrations. Despite differences in their growth responses with respect to oxygen, we found a clear correlation between pO(2) and magnetosome formation in all three Magnetospirillum strains. Magnetite biomineralization was induced only below a threshold value of 20 mbar O(2) and optimum conditions for magnetosome formation were found at a pO(2) of 0.25 mbar (1 bar = 10(5) Pa). A maximum yield of 6.3 mg magnetite l(-1) day(-1) was obtained with M. gryphiswaldense grown under oxystat conditions, which is the highest magnetosome productivity reported so far for a magnetotactic bacterium. In conclusion, the presented results provide the basis for large-scale cultivation of magnetospirilla under defined conditions.

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Citations

Mar 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Arash KomeiliDianne K Newman
Nov 23, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sarah StanilandNeil Telling
Jun 19, 2008·Journal of the Royal Society, Interface·Atsushi ArakakiTadashi Matsunaga
Dec 28, 2012·Journal of the Royal Society, Interface·Andreas U GehringInés García-Rubio
Sep 8, 2006·Applied and Environmental Microbiology·Sabrina SchübbeDirk Schüler
Jun 10, 2008·Applied and Environmental Microbiology·Claus Lang, Dirk Schüler
May 26, 2009·Applied and Environmental Microbiology·Sabrina SchübbeDennis A Bazylinski
Apr 28, 2009·Applied and Environmental Microbiology·Claus LangDirk Schüler
Jul 19, 2011·Applied and Environmental Microbiology·Anna PollithyDirk Schüler
Feb 10, 2004·Applied and Environmental Microbiology·Karen GrünbergDirk Schüler
Jul 2, 2014·PloS One·Mathieu BennetDamien Faivre
Jul 6, 2012·Applied Microbiology and Biotechnology·Wei ZhouHong Peng
Sep 13, 2013·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Edouard AlphandéryMickaël Durand-Dubief
Dec 15, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Lei YanHongyu Li
Aug 10, 2007·Environmental Microbiology·Sheri L SimmonsKatrina J Edwards
May 18, 2010·Journal of Basic Microbiology·Sarah S StanilandLiane G Benning
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Oct 27, 2011·Molecular Microbiology·Emanuel KatzmannDirk Schüler
May 25, 2011·Environmental Microbiology·Christopher T LefèvreDennis A Bazylinski

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