Growth and Quantification of MERS-CoV Infection

Current Protocols in Microbiology
Christopher M Coleman, Matthew B Frieman

Abstract

Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging highly pathogenic respiratory virus. Although MERS-CoV only emerged in 2012, we and others have developed assays to grow and quantify infectious MERS-CoV and RNA products of replication in vitro. MERS-CoV is able to infect a range of cell types, but replicates to high titers in Vero E6 cells. Protocols for the propagation and quantification of MERS-CoV are presented.

References

May 13, 2006·The Journal of General Virology·Alexander O PasternakEric J Snijder
Feb 12, 2008·Current Protocols in Molecular Biology·Mary C Phelan
Dec 5, 2008·Microbiology and Molecular Biology Reviews : MMBR·Matthew Frieman, Ralph Baric
Oct 19, 2012·The New England Journal of Medicine·Ali M ZakiRon A M Fouchier
Sep 13, 2013·Journal of Virology·Daniela NiemeyerMarcel A Müller
Sep 26, 2013·Proceedings of the National Academy of Sciences of the United States of America·Emmie de WitVincent J Munster
Nov 8, 2013·The Journal of General Virology·Christopher M ColemanMatthew B Frieman
Aug 22, 2014·PLoS Pathogens·Darryl FalzaranoVincent J Munster

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Citations

Mar 24, 2017·Scientific Reports·Sawsan S Al-AmriAnwar M Hashem
Nov 1, 2016·Journal of Virology·Christopher M ColemanMatthew B Frieman
Feb 19, 2016·Science Translational Medicine·Thomas LukeMatthew B Frieman
Jun 25, 2020·Science Advances·Neeltje van DoremalenVincent J Munster
Jan 5, 2021·PeerJ·Nicole BracciShih-Chao Lin
Nov 27, 2020·Frontiers in Cell and Developmental Biology·Desh Deepak SinghDharmendra K Yadav
May 11, 2021·Nature·Kevin O SaundersBarton F Haynes

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Datasets Mentioned

BETA
KC776174.1

Methods Mentioned

BETA
Assay
PCR

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