Growth and repair factors, osteoactivin, matrix metalloproteinase and heat shock protein 72, increase with resolution of inflammation in musculotendinous tissues in a rat model of repetitive grasping

BMC Musculoskeletal Disorders
Nagat FraraMary F Barbe

Abstract

Expression of the growth factor osteoactivin (OA) increases during tissue degeneration and regeneration, fracture repair and after denervation-induced disuse atrophy, concomitant with increased matrix metalloproteinases (MMPs). However, OA's expression with repetitive overuse injuries is unknown. The aim of this study was to evaluate: 1) OA expression in an operant rat model of repetitive overuse; 2) expression of MMPs; 3) inflammatory cytokines indicative of injury or inflammation; and 4) the inducible form of heat shock protein 70 (HSPA1A/HSP72) as the latter is known to increase during metabolic stress and to be involved in cellular repair. Young adult female rats performed a high repetition negligible force (HRNF) food retrieval task for up to 6 weeks and were compared to control rats. Flexor digitorum muscles and tendons were collected from 22 young adult female rats performing a HRNF reaching task for 3 to 6 weeks, and 12 food restricted control (FRC) rats. OA mRNA levels were assessed by quantitative polymerase chain reaction (qPCR). OA, MMP-1, -2, -3, and -13 and HSP72 protein expression was assayed using Western blotting. Immunohistochemistry and image analysis was used to evaluate OA and HSP72 expression. ELISA was pe...Continue Reading

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Citations

Jul 12, 2016·Expert Opinion on Therapeutic Targets·Yuyang HuangYongchang Yao
Jul 14, 2018·The British Journal of Radiology·Magnus Borga
Dec 16, 2019·British Medical Bulletin·Emanuele ChisariNicola Maffulli
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Oct 9, 2020·Connective Tissue Research·Brendan A HilliardMary F Barbe
Jul 30, 2021·Acta Biomaterialia·Patrick M WilliamsonAra Nazarian

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Methods Mentioned

BETA
PCR
PCT
electrophoresis
ELISAs
ELISA

Software Mentioned

Bioquant Osteo
Image J
GraphPad
Prism
myImageAnalysis

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