Growth arrest and decrease of alpha-SMA and type I collagen expression by palmitic acid in the rat hepatic stellate cell line PAV-1.

Digestive Diseases and Sciences
Armand AbergelPatrick Sauvant

Abstract

Liver fibrosis is characterized by an activation of hepatic stellate cells (HSC). During primary culture HSC evolve from a quiescent into an activated phenotype which is characterized by alpha-smooth muscle actin (alpha-SMA) up-regulation, increase in cell growth, and extracellular matrix secretion. HSC culture with trans-resveratrol can lead to deactivation of myofibroblast-like HSC. We used an HSC line, PAV-1, to check the role of retinol and palmitic acid in the deactivation process of HSC. Using mass and metabolic-based methods, Western blot and immunocytochemistry assays, we demonstrated that treatment with palmitic acid (75 muM) alone or in combination with retinol (2 muM) significantly decreased cell proliferation and alpha-SMA expression. We also established that the association of both compounds strongly decreased collagen type I expression. Our results suggest the potential use of palmitic acid alone or in combination with retinol to induce HSC deactivation.

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Citations

Jun 1, 2011·Journal of Physiology and Biochemistry·Patrick SauvantClaude Atgié
Aug 1, 2008·Molecular Biology of the Cell·Gunter MaubachLang Zhuo
Apr 8, 2010·Journal of Gastroenterology and Hepatology·Monique AllmanChantal A Rivera
Jun 1, 2010·BMC Gastroenterology·Chantal A RiveraKevin Pruitt
Mar 4, 2014·Hepatology International·Michal Ganz, Gyongyi Szabo
Jun 7, 2015·Archives of Toxicology·Sara Crespo YanguasMathieu Vinken
Apr 13, 2007·Journal of Applied Toxicology : JAT·Enrique ChávezPablo Muriel
Sep 19, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Kouichi MiuraEkihiro Seki
Nov 5, 2015·Molecular Medicine Reports·Hui ZhangJiguang Ding
Aug 16, 2017·Cancer Biology & Therapy·Juhong DengJiaquan Huang

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