PMID: 11341986May 9, 2001Paper

Growth arrest in A549 cells during hyperoxic stress is associated with decreased cyclin B1 and increased p21(Waf1/Cip1/Sdi1) levels

Biochimica Et Biophysica Acta
S A McGrath-Morrow, J Stahl

Abstract

Exposure to high concentrations of oxygen has previously been shown to cause growth arrest in A549 cells, a distal lung epithelial cell line. We found that when A549 cells were exposed to 95% oxygen they underwent substantial growth inhibition. This was associated with induction of p21(Waf1/Cip1/Sdi1) protein and a decrease in cyclin B1 protein. Flow cytometry revealed that A549 cells exposed to hyperoxia had a significant decrease in the percentage of cells in G(1) and a modest but significant increase in the percentage of cells in S phase and G(2)/M, consistent with cells entering S phase. A549 cells in room air and hyperoxia were then treated with nocodazole, a mitotic inhibitor. Room air A549 cells treated with nocodazole showed a marked increase in G(2)/M consistent with mitotic arrest. In contrast, hyperoxic treated cells had a modest but significant decrease in G(1) but only a minimal increase in G(2)/M consistent with partial G(1)/S arrest and growth inhibition in S phase. To further investigate the role of p21(Waf1/Cip1/Sdi1) as a checkpoint regulator during hyperoxic growth inhibition, HCT116 cells with wild-type and null p21(Waf1/Cip1/Sdi1) were exposed to hyperoxia. Both wild-type p21(+/+) cells and null p21(-/-) ce...Continue Reading

References

Jun 3, 1988·Science·J A Imlay, S Linn
Jan 1, 1986·Annual Review of Physiology·J D Crapo
Jan 1, 1997·Advances in Cancer Research·S E Morgan, M B Kastan
Aug 1, 1997·Molecular and Cellular Biology·V V OgryzkoB H Howard
Feb 26, 1998·American Journal of Respiratory Cell and Molecular Biology·S A McGrath
Nov 7, 1998·American Journal of Respiratory Cell and Molecular Biology·M A O'ReillyW M Maniscalco
May 1, 1999·Biochemical and Biophysical Research Communications·M C StubbsD J Hall
Aug 26, 1999·American Journal of Respiratory Cell and Molecular Biology·J S Shenberger, P S Dixon
Jun 15, 2000·Experimental Cell Research·C P De SouzaB G Gabrielli

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Citations

Nov 18, 2010·In Vitro Cellular & Developmental Biology. Animal·Hong-Yan LuHong Wang
Apr 3, 2003·Pulmonary Pharmacology & Therapeutics·Rubin M TuderEnid Neptune
Jan 10, 2004·Antioxidants & Redox Signaling·Kumuda C Das, Dashnamoorthy Ravi
Nov 23, 2006·Antioxidants & Redox Signaling·Stefan W RyterAugustine M K Choi
Jan 24, 2007·Bioinformatics·Yanxin ShiZiv Bar-Joseph
Sep 22, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Meng ZhangYang Jin
Dec 6, 2001·American Journal of Respiratory and Critical Care Medicine·A ClementS Corroyer
Mar 29, 2014·American Journal of Respiratory Cell and Molecular Biology·Hataya K PoonyagariyagornEnid R Neptune
Dec 1, 2005·European Journal of Pharmacology·Teresa Sanchez, Juan J Moreno
Dec 13, 2005·Free Radical Biology & Medicine·Emilie BoncoeurAlexandra Henrion-Caude
Mar 3, 2004·Biochimica Et Biophysica Acta·Folasade OgunlesiSharon A McGrath-Morrow
Jul 6, 2014·Biochemical and Biophysical Research Communications·Kirti Kumar TiwariKrithika Lingappan
May 15, 2008·Pediatric Critical Care Medicine : a Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies·Yan ZhuAaron Chidekel
Sep 10, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kumuda C Das, Ravi Dashnamoorthy

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