PMID: 8580912Dec 1, 1995Paper

Growth-blocking peptide titer during larval development of parasitized and cold-stressed armyworm

Insect Biochemistry and Molecular Biology
A OhnishiS Sekiguchi

Abstract

Growth-blocking peptide (GBP) has been isolated for the first time from the haemolymph of the host armyworm Pseudaletia separata whose development was halted in the last larval instar stage by parasitization with the parasitoid wasp Cotesia kariyai. Recent studies demonstrated that GBP not only exists in the plasma (haemolymph without cells) of parasitized last instar larvae, but also in the plasma of nonparasitized penultimate (5th) instar larvae. Monoclonal antibodies were prepared to measure the titers of GBP in nonparasitized and parasitized larval plasma. One of three monoclonal antibodies raised against GBP, which is the most specific for GBP, was used to quantify the concentration of plasma GBP. As this antibody recognized two plasma peptides other than GBP in crude plasma fractions, each plasma peptide fraction was separated by a reversed phase HPLC, and then plasma GBP level was measured by ELISA. The highest level of plasma GBP detected on Day 0 of the penultimate instar larvae was gradually decreased throughout the larval growth except for the temporary increase on Day 0 of last larval instar. After parasitization on Day 0 of last larval instar, two peaks of plasma GBP titer were detected during the last larval insta...Continue Reading

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Citations

Sep 13, 2002·Insect Biochemistry and Molecular Biology·M D Lavine, M R Strand
Feb 13, 2001·European Journal of Biochemistry·H Noguchi, Y Hayakawa
Aug 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yasunori OdaYoichi Hayakawa
Aug 28, 2009·The Journal of Biological Chemistry·Yoshitaka UmetsuKeiichi Kawano
Dec 27, 2011·Insect Biochemistry and Molecular Biology·Koichiro YamaguchiYoichi Hayakawa
May 17, 2005·Journal of Insect Physiology·Kevin D ClarkMichael R Strand
Jan 18, 2005·Mechanisms of Development·Seiji TsuzukiYoichi Hayakawa
Dec 3, 2013·Journal of Insect Physiology·Hikaru KiyotakeYoichi Hayakawa
Jul 29, 2016·PloS One·Shunsuke FurihataYoichi Hayakawa
Jan 16, 1999·The Journal of Biological Chemistry·T AizawaK Nitta
Mar 26, 2003·Archives of Insect Biochemistry and Physiology·Yuichi NakaharaManabu Kamimura
Apr 11, 2019·Frontiers in Physiology·Stephen B Shears, Yoichi Hayakawa
Oct 1, 1998·Biochemical and Biophysical Research Communications·Y Hayakawa, A Ohnishi

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