Growth characteristics of two rhinovirus strains in WI-26 and monkey kidney cells.

Journal of Bacteriology
R F HaffR C Stewart

Abstract

Haff, R. F. (Smith Kline & French Laboratories, Philadelphia, Pa.), B. Wohlsen, E. E. Force, and R. C. Stewart. Growth characteristics of two rhinovirus strains in WI-26 and monkey kidney cells. J. Bacteriol. 91:2339-2342. 1966.-Viruses with 1059 and HGP serotype and with human and monkey host range characteristics, respectively, were employed. Adsorption kinetics of the 1059 and HGP strains to WI-26 cells, and HGP to Green African monkey kidney cells (MKC), were similar. Fifty per cent of the virus was adsorbed to cell monolayers within 10 min; adsorption was essentially complete by 2 hr. The 1059 strain failed to adsorb to MKC, at least to an appreciable extent. Lack of receptors for adsorption of 1059 accounts for the inability of this cell to support multiplication of the virus. It is probable that MKC are refractory to infection with other H strains of rhinovirus for the same reason. Single-step multiplication cycles have been described for the HGP strain in WI-26 and MKC cultures and for the 1059 strain in WI-26 cells. In both cells, HGP exhibited a latent period of 7 hr. Increase of intracellular and cell-associated virus appeared somewhat prior to that of extracellular virus. Maximal titers were attained by 9 to 10 hr. ...Continue Reading

References

Aug 1, 1961·The Journal of Experimental Medicine·J J HOLLAND, L C McLAREN
Feb 11, 1961·British Medical Journal·D A TYRRELL, M L BYNOE

❮ Previous
Next ❯

Citations

Jan 1, 1968·Archiv für die gesamte Virusforschung·R D Fletcher, D Platt
Aug 1, 1984·Journal of Virology·G Abraham, R J Colonno

❮ Previous
Next ❯

Related Concepts

Related Feeds

Acute viral rhinopharyngitis

Acute viral rhinopharyngitis, also known as "common cold", is an acute, self-limiting viral infection of the upper respiratory tract involving the nose, sinuses, pharynx and larynx. Discover the latest research on acute viral rhinopharyngitis here.