Growth condition-dependent cell surface proteome analysis of Enterococcus faecium

Proteomics
Jan C SinnigeJanetta Top

Abstract

The last 30 years Enterococcus faecium has become an important nosocomial pathogen in hospitals worldwide. The aim of this study was to obtain insight in the cell surface proteome of E. faecium when grown in laboratory and clinically relevant conditions. Enterococcus faecium E1162, a clinical blood stream isolate, was grown until mid-log phase in brain heart infusion medium (BHI) with, or without 0.02% bile salts, Tryptic Soy Broth with 1% glucose (TSBg) and urine, and its cell surface was "shaved" using immobilized trypsin. Peptides were identified using MS/MS. Mapping against the translated E1162 whole genome sequence identified 67 proteins that were differentially detected in different conditions. In urine, 14 proteins were significantly more and nine proteins less abundant relative to the other conditions. Growth in BHI-bile and TSBg, revealed four and six proteins, respectively, which were uniquely present in these conditions while two proteins were uniquely present in both conditions. Thus, proteolytic shaving of E. faecium cells identified differentially surface exposed proteins in different growth conditions. These proteins are of special interest as they provide more insight in the adaptive mechanisms and may serve as ...Continue Reading

References

Oct 24, 1997·Science·R L TatusovD J Lipman
May 7, 2003·Current Microbiology·Marinella Silva LaportMarcia Giambiagi-deMarval
Sep 13, 2003·BMC Bioinformatics·Roman L TatusovDarren A Natale
Oct 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·K A WALSH, H NEURATH
Oct 12, 2004·Journal of Proteome Research·Lewis Y GeerStephen H Bryant
Jun 21, 2005·Emerging Infectious Diseases·Rob J L WillemsMarc J M Bonten
Apr 5, 2007·Nature Protocols·Andrej ShevchenkoMatthias Mann
Sep 11, 2007·Journal of Bacteriology·Esther HeikensRob J L Willems
Mar 29, 2008·BMC Bioinformatics·Miaomiao ZhouRoland J Siezen
Apr 24, 2008·Molecular Microbiology·Girbe BuistOscar P Kuipers
Oct 4, 2008·Microbiology·Antoni P A HendrickxRob J L Willems
Aug 12, 2009·Antimicrobial Agents and Chemotherapy·Cesar A AriasBarbara E Murray
Sep 4, 2009·Trends in Microbiology·Antoni P A HendrickxWillem van Schaik
Jul 28, 2010·Proteomics·Annette DreisbachJan Maarten van Dijl
Mar 3, 2011·BMC Genomics·Liv Anette BøhleGeir Mathiesen
Jun 2, 2011·Proteomics·Annette DreisbachGirbe Buist
Mar 17, 2012·Nature Reviews. Microbiology·Cesar A Arias, Barbara E Murray
Dec 4, 2012·Nucleic Acids Research·Juan Antonio VizcaínoHenning Hermjakob
Apr 30, 2013·Journal of Proteomics·Alfonso Olaya-AbrilManuel J Rodríguez-Ortega
Aug 21, 2013·Microbiology·Charlotte MichauxJean-Christophe Giard
Jan 7, 2014·Journal of Proteome Research·Thomas PribylSven Hammerschmidt

❮ Previous
Next ❯

Citations

Sep 15, 2019·Journal of Separation Science·Yanan LiMingliang Ye
Nov 12, 2019·Frontiers in Microbiology·Kate L HarveySteven P Djordjevic

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Enfermedades infecciosas y microbiología clínica
M Carmen Fariñas, Carmen Torres
Journal of Separation Science
Pawel Olszowy, Boguslaw Buszewski
JAMA : the Journal of the American Medical Association
R V Spera, B F Farber
© 2021 Meta ULC. All rights reserved