Growth conditions of Aspergillus sp. ATHUM-3482 for polygalacturonase production

Applied Microbiology and Biotechnology
M Galiotou-PanayotouO Kalantzi

Abstract

A wild type of Aspergillus sp. ATHUM-3482 produced extracellular polygalacturonase when grown in liquid medium containing citrus pectin as sole carbon source. A number of factors affecting enzyme activity were investigated. Polygalacturonase activities as high as 4.3 U ml-1 (reducing-group-releasing activity) and 17 U ml-1 (viscosity-diminishing activity) were obtained under optimum growth conditions. With sugar-beet as sole carbon source the respective activities were 6.5 U ml-1 and 40 U ml-1, the highest achieved in this work. Under these conditions no pectin lyase or pectinesterase activity was detected. The above yields of polygalacturonase activity compare favourably with those reported for fungi grown under similar growth conditions.

Citations

May 6, 2008·Applied Biochemistry and Biotechnology·Danielle Biscaro PedrolliEleonora Cano Carmona
Feb 7, 2008·Journal of Industrial Microbiology & Biotechnology·Maria do Rosário FreixoJosé Maria Arteiro
Sep 28, 2013·Revista iberoamericana de micología·Sofía Borrego Alonso, Ivette Perdomo Amistad
Apr 4, 2014·Applied Microbiology and Biotechnology·Doreen HeerdMarcelo Fernández-Lahore
May 27, 2009·Bioresource Technology·Roselei Claudete FontanaMauricio Moura da Silveira
Apr 28, 2017·Journal of Molecular Microbiology and Biotechnology·Dante FratebianchiSebastián Fernando Cavalitto
Sep 1, 2020·Journal, Genetic Engineering & Biotechnology·Fatima AslamHaneef Ur Rehman

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