Growth-differentiation factor-8 (GDF-8) in the uterus: its identification and functional significance in the golden hamster.

Reproductive Biology and Endocrinology : RB&E
Chun Lung WongPak Ham Chow

Abstract

Transforming growth factor-beta superfamily regulates many aspects of reproduction in the female. We identified a novel member of this family, growth-differentiation factor 8 (GDF-8) in the 72 h post coital uterine fluid of the golden hamster by proteomic techniques. Uterine GDF-8 mRNA decreased as pregnancy progressed while its active protein peaked at 72 h post coitus (hpc) and thereafter stayed at a lower level. At 72 hpc, the GDF-8 transcript was localized to the endometrial epithelium while its protein accumulated in the stroma. Exogenous GDF-8 slowed down proliferation of primary cultures of uterine smooth muscle cells (SMC) and endometrial epithelial cells (EEC). In addition, GDF-8 attenuated the release of LIF (leukaemia inhibiting factor) by EEC. As for the embryo in culture, GDF-8 promoted proliferation of the trophotoderm (TM) and hatching but discouraged attachment. Our study suggests that GDF-8 could regulate the behavior of preimplantation embryos and fine-tune the physiology of uterine environment during pregnancy.

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Citations

Jul 24, 2012·Placenta·H N Peiris, M D Mitchell
Oct 30, 2018·Journal of Cellular Physiology·Susana Fernández-NoceloVíctor M Arce
Jan 1, 2012·Animals : an Open Access Journal From MDPI·Leo O Fiems

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Methods Mentioned

BETA
electrophoresis
PCR
ELISA
FCS

Software Mentioned

MASCOT
MS
ABI SDS
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Motic Images Plus
Metamorph

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