Growth factor control of cultured rat uterine stromal cell proliferation is progesterone dependent

Biology of Reproduction
M PivaV Rider

Abstract

Uterine stromal cells undergo mitosis and differentiate into the decidua just prior to the expected time of implantation in humans and rodents. We have utilized a culture system that will be suitable for study of the molecular mechanisms regulating stromal cell proliferation. Stromal cells were isolated from the uteri of ovariectomized rats and were cultured in chemically defined medium. Cultured cells express the mesenchymal markers vimentin and desmin. They do not express the epithelial marker cytokeratin. Serum-starved stromal cells were stimulated to proliferate in a time frame consistent with the cell cycle through addition of a panel of growth factors (basic fibroblast growth factor [bFGF], epidermal growth factor, platelet-derived growth factor, transforming growth factor alpha, insulin-like growth factor I) and hormones to the culture medium. None of the growth factors tested significantly stimulated proliferation in the absence of progesterone. Furthermore, progesterone was the only steroid of those tested that stimulated mitosis in the presence of growth factors. Stromal cell proliferation in response to progesterone and bFGF was dose dependent and saturable. Addition of the progesterone receptor antagonist mifepristo...Continue Reading

Citations

Apr 11, 2013·Journal of Molecular Histology·Zhi-Kun BaiZhan-Peng Yue
Feb 26, 2000·European Journal of Obstetrics, Gynecology, and Reproductive Biology·A G GuninD V Nikolaev
May 10, 2008·The Journal of Biological Chemistry·S M Khorshed AlamMichael J Soares
Sep 3, 2011·American Journal of Reproductive Immunology : AJRI·Alexandre U BorbelyEstela Bevilacqua
May 4, 2004·The Journal of Steroid Biochemistry and Molecular Biology·H WelterR Einspanier
Mar 16, 2016·The Journal of Endocrinology·Virginia RiderBruce F Kimler
Aug 26, 1998·Biology of Reproduction·V RiderW M Justice
Jun 20, 2002·Endocrinology·Shaw-Jenq TsaiLih-Yuh C Wing

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