Dec 17, 2009

Growth factor delivery through electrospun nanofibers in scaffolds for tissue engineering applications

Journal of Biomedical Materials Research. Part a
S SahooSiew Lok Toh

Abstract

Tissue engineering scaffolds should ideally mimic the natural ECM in structure and function. Electrospun nanofibrous scaffolds are easily fabricated and possess a biomimetic nanostructure. Scaffolds can mimic ECM function by acting as a depot for sustained release of growth factors. bFGF, an important growth factor involved in tissue repair and mesenchymal stem cell proliferation and differentiation, is a suitable candidate for sustained delivery from scaffolds. In this study, we present two types of PLGA nanofibers incorporated with bFGF, fabricated using the facile technique of blending and electrospinning (Group I) and by the more complex technique of coaxial electrospinning (Group II). bFGF was randomly dispersed in Group I and distributed as a central core within Group II nanofibers; both scaffolds showed similar protein encapsulation efficiency and release over 1-2 weeks. Although both scaffold groups favored bone marrow stem cell attachment and subsequent proliferation, cells cultured on Group I scaffolds demonstrated increased collagen production and upregulated gene expression of specific ECM proteins, indicating fibroblastic differentiation. The study shows that the electrospinning technique could be used to prolong g...Continue Reading

  • References36
  • Citations28

References

Mentioned in this Paper

Biomimetics
FGF2 gene
Extracellular Matrix
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression
Cell Proliferation
Cell Differentiation Process
Zyderm
Recombinant Fibroblast Growth Factor-2
Growth Factor

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