Growth factor dependence of pheochromocytoma cells in chemically defined medium

Journal of Neuroscience Research
R Goodman, H Herschman

Abstract

We have previously described the isolation of a clonal pheochromocytoma cell line (PC-G2) which responds to nerve growth factor (NGF) [Goodman and Herschman, 1978] and epidermal growth factor (EGF) [Goodman et al, 1980] with increased specific activity of tyrosine hydroxylase (TH). This cell line thus can be used for studies of induction of a key enzyme in the biosynthesis of catecholamines using a homogeneous cell population under controlled environmental influences. However, the need for serum in the culture medium used to grow these cells still introduced a lack of total environmental control. Several laboratories have circumvented this problem by growing cells in chemically defined medium. In the present report we demonstrate that with a modification PC-G2 cells can survive and grow in the chemically defined N3 medium devised by Bottenstein and Sato [1979]. While another pheochromocytoma cell line, PC-12, was shown to grow in N3 medium without any supplement [Bottenstein et al, 1979], the PC-G2 cells exhibit an absolute requirement for NGF or EGF in order to grow in this chemically defined medium. As was the case in the induction of TH in serum-containing medium [Goodman and Herschman, 1978; Goodman et al. 1980], EGF was fo...Continue Reading

References

Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R Goodman, H R Herschman
Jan 1, 1978·Annual Review of Biochemistry·R A Bradshaw
Feb 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·R Levi-MontalciniH Thoenen
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J E Bottenstein, G H Sato
Jan 1, 1978·Journal of Supramolecular Structure·H R HerschmanA Aharonov
Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·L A Greene, A S Tischler
Oct 1, 1972·Journal of Cellular Physiology·A Sivak
May 15, 1974·International Journal of Cancer. Journal International Du Cancer·L DiamondY Shimizu
Feb 1, 1972·Cancer·S Warren, R N Chute

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