Growth factor-independent proliferation of rat mammary carcinoma cells by autocrine secretion of neu-differentiation factor/heregulin and transforming growth factor-alpha

Molecular Carcinogenesis
S P EthierC A Dilts

Abstract

Serially transplantable rat mammary tumor (RMT) cells are not dependent on exogenous epidermal growth factor (EGF) and insulin-like growth factor-I for continuous growth in serum-free medium. Previously, we found that conditioned medium obtained from these cells contained EGF-like mitogenic activity and stimulated tyrosine phosphorylation of a 185-kDa protein in EGF-dependent mammary epithelial cells. This protein is distinct from the EGF receptor and resembles a 185-kDa tyrosine-phosphorylated protein present in RMT cells themselves. The results of the studies reported here indicate that the tyrosine-phosphorylated p185 detected in growth factor-independent RMT cells and in human mammary epithelial cells exposed to RMT-conditioned medium was activated erbB-2 protein. Partial purification of the activating factor present in RMT-conditioned medium yielded a heparin-binding growth factor with biochemical properties similar to those of neu differentiation factor/heregulin (NDF/HRG). RNA-polymerase chain reaction analysis demonstrated that RMT cells expressed mRNA for NDF/HRG, and western-blot analysis confirmed the presence of the 45-kDa secreted form of NDF/HRG in conditioned medium from the growth factor-independent RMT cells. T...Continue Reading

References

May 1, 1991·Breast Cancer Research and Treatment·S P Ethier, R Moorthy
Oct 1, 1990·The Journal of Investigative Dermatology·E FinziJ H Pierce
Nov 15, 1987·International Journal of Cancer. Journal International Du Cancer·U RodeckH Koprowsk
Jul 5, 1995·Journal of the National Cancer Institute·S P Ethier

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Citations

May 14, 2011·The Journal of Biological Chemistry·Sanjeev KumarDavid A Isenberg
Oct 7, 2005·Neoplasia : an International Journal for Oncology Research·Kathleen M Woods IgnatoskiStephen P Ethier
Mar 6, 2010·Molecular and Cellular Biochemistry·Sutapa MukhopadhyaySalil K Das
Feb 17, 2006·Molecular Carcinogenesis·M SchmittR P DiAugustine
Jan 24, 2004·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Murthy GollamudiJeffrey A Kern

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