Growth factor-induced transcription via the serum response element is inhibited by cyclic adenosine 3',5'-monophosphate in MCF-7 breast cancer cells

Endocrinology
W L LoweM Banko

Abstract

The effect of increased intracellular cAMP on MCF-7 breast cancer cell growth was examined by treating cells with either forskolin, an activator of adenylate cyclase, or 8-[4-chlorophenylthio]-cAMP (8-CPT-cAMP), a cAMP analog. Compared to cells maintained in control medium, treatment with either 1 or 10 microM forskolin decreased cell growth by 17% and 68%, respectively, whereas treatment with 250 microM 8-CPT-cAMP decreased cell growth by 29%. To determine whether this effect of cAMP on cell growth was mediated by inhibition of the activity of extracellular signal-regulated kinases 1 and 2 (ERK1 and -2), two mitogen-activated protein kinases, the effect of cAMP on growth factor-induced ERK activity in MCF-7 cells was examined. Treatment with either insulin-like growth factor I (IGF-I) or epidermal growth factor (EGF) for 10 min stimulated a 4- to 8-fold increase in ERK1 and -2 activity. This effect of IGF-I and EGF was not inhibited by increased intracellular cAMP generated by pretreatment of the cells with 10 microM forskolin. Similarly, 10 microM forskolin had no effect on IGF-I- or EGF-induced ERK activity in cells treated with growth factor for 30 min. To determine whether cAMP inhibits other growth factor-mediated effects...Continue Reading

References

May 1, 1992·Biochemical Society Transactions·Y S Cho-Chung
Nov 1, 1992·Cell Proliferation·F RevillionJ Lefebvre
Jan 1, 1992·Advances in Cancer Research·K Seuwen, J Pouysségur
Oct 1, 1992·Trends in Biochemical Sciences·R Treisman
Dec 25, 1992·Biochemical and Biophysical Research Communications·W L Lowe, R M Teasdale
Dec 1, 1991·Baillière's Clinical Endocrinology and Metabolism·D R Brigstock
Nov 1, 1989·The Journal of Clinical Investigation·C L ArteagaC K Osborne
Oct 15, 1985·International Journal of Cancer. Journal International Du Cancer·L G Sheffield, C W Welsch
Aug 1, 1987·Molecular and Cellular Biology·C Chen, H Okayama
May 1, 1983·Cell Biology International Reports·W KüngU Eppenberger
Jan 1, 1994·Breast Cancer Research and Treatment·D Yee
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L M GravesE G Krebs
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B R SevetsonJ C Lawrence
Nov 12, 1993·Science·S J Cook, F McCormick
Jan 1, 1994·Breast Cancer Research and Treatment·N NormannoD S Salomon
Jan 1, 1994·Breast Cancer Research and Treatment·J Baselga, J Mendelsohn
Jul 29, 1994·Biochemical and Biophysical Research Communications·Y NagasakaT Kaneko
Feb 1, 1994·Current Opinion in Genetics & Development·R Treisman
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·G PagèsJ Pouysségur
Oct 1, 1995·Endocrine Reviews·R B Dickson, M E Lippman

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Citations

Jan 12, 2005·Breast Cancer Research : BCR·Dragoslava ZivadinovicCheryl S Watson
Feb 23, 2002·European Journal of Pharmacology·Caroline NunnDaniel Hoyer
Nov 24, 1999·Pharmacology & Toxicology·L P Niles, J Wang
Sep 23, 2000·American Journal of Physiology. Endocrinology and Metabolism·W LiuW L Lowe

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