PMID: 9450679Feb 5, 1998Paper

Growth factor induction of nitric oxide synthase in rat pheochromocytoma cells

Brain Research. Molecular Brain Research
A M SheehyS M Black

Abstract

Previous work has suggested that nerve growth factor treatment of PC12 cells induces neuronal nitric oxide synthase, and possibly also endothelial nitric oxide synthase (NOS) and inducible NOS. To further analyze this process we exposed rat pheochromocytoma (PC12) cells to increasing concentrations of basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), nerve growth factor (NGF), and vascular endothelial cell growth factor (VEGF). Changes in NOS expression were then analyzed by Western blotting, using antisera generated against the three isoforms of NOS. Our results demonstrate that neuronal NOS was induced by growth factors that promote both differentiation (bFGF, NGF) and proliferation (EGF). nNOS levels were unaffected by VEGF treatment. In contrast, the levels of endothelial and inducible NOS were undetectable in these same cells, suggesting that different clonal lines of PC12 cells have different isoform complements.

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Citations

Feb 17, 2001·Journal of Cellular Biochemistry·D A BulsecoA H Ross
Nov 30, 2012·Cellular and Molecular Neurobiology·Tomas R GranaBettina E Kalisch
Aug 23, 2005·Brain Research. Brain Research Reviews·Esperanza R MatarredonaCarmen Estrada
Mar 27, 2004·European Journal of Pharmacology·Matsumi YamazakiHiroshi Hatanaka
Jul 21, 2001·The Biochemical Journal·J MwanjeweA K Grover
Mar 10, 2001·Journal of Neuroimmunology·M T Heneka, D L Feinstein
Jan 18, 2006·The Journal of Steroid Biochemistry and Molecular Biology·Vasilia-Ismini AlexakiElias Castanas
Oct 22, 2005·Neuroscience Letters·Matsumi YamazakiTetsuro Mohri
Aug 16, 2005·Brain Research. Developmental Brain Research·Mark S WainwrightStephen M Black
Aug 23, 2005·British Journal of Pharmacology·Matsumi YamazakiTetsuro Mohri
Mar 24, 2015·Neuroscience Letters·Megan R Strachan-WhaleyBettina E Kalisch
Jul 27, 2001·Veterinary Pathology·T SakoH Taniyama
Mar 27, 2004·American Journal of Physiology. Cell Physiology·Jingying Sarah ZhangGeorge A Truskey
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·D C SkinnerA Caraty
Jul 4, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·S WedgwoodS M Black
Jun 5, 1998·The American Journal of Physiology·A M SheehyS M Black

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