Growth factor receptor expression during in vitro differentiation of partially purified populations containing murine stem cells

Journal of Cellular Physiology
E AshiharaA R Migliaccio

Abstract

We have investigated, by semiquantitative RT-PCR, the kinetics of activation of hematopoietic receptors and differentiation markers in partially purified murine hematopoietic stem cells (HSC) induced to differentiate in serum-free culture with combinations of growth factor (GF). The combinations of GF used sustained either multilineage [stem cell factor (SCF) + interleukin 3 (IL-3), or erythroid [SCF + IL-3 + erythropoietin (Epo)] or myeloid [SCF + IL-3 + granulocyte colony-stimulating factor (G-CSF)] differentiation. The GF receptor genes investigated were the alpha and beta subunits of the IL-3 and granulocyte-macrophage colony-stimulating factor (GM-CSF) receptor, the erythropoietin receptor, the G-CSF receptor, and c-Fms, the receptor for macrophage colony-stimulating factor (M-CSF). The expression of Gata1 and alpha- and beta-globin was investigated at the same time as a marker of erythroid differentiation. HSC were purified according to standard protocols, which include partitioning of lineage-negative bone marrow cells with the mitochondrial dye Rhodamine 123 (Rho) into Rho-dull (> or = 17% of which reconstitute long-term hematopoiesis in recipient mice) and into Rho-bright (which are as capable as Rho-dull of multilinea...Continue Reading

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Citations

Aug 1, 2012·Biologics : Targets & Therapy·Steve Elliott, Angus M Sinclair
Sep 11, 2003·Journal of Hematotherapy & Stem Cell Research·Anna Rita MigliaccioGiovanni Migliaccio
Mar 8, 2003·Stroke; a Journal of Cerebral Circulation·W-R SchäbitzS Schwab
May 20, 1998·Biotherapy·A R Migliaccio, G Migliaccio
Jun 17, 2005·Annals of the New York Academy of Sciences·Anna Rita MigliaccioFrancesco A Manzoli
May 26, 2005·Blood·Kazuhiro MakiKinuko Mitani

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