PMID: 3759796Sep 1, 1986Paper

Growth factor responses of human arterial endothelial cells in vitro

In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association
R Weinstein, K Wenc

Abstract

Human arterial endothelial cells were cultured in vitro for up to 40 cumulative population doublings. Culture conditions similar to those required for long-term propagation of human umbilical vein endothelial cells were employed. These included fibronectin-coated culture vessels, 5 to 20% fetal bovine serum, endothelial cell growth factor, and heparin. Thoracic aorta endothelial cells were larger than iliac artery endothelial cells. Both cell types stained positively for Factor VIII antigen by immunofluorescence. A decrease in confluent density as a function of population doubling level was correlated with the appearance of large, senescent cells in the cultures. Serum growth factors to which the arterial endothelial cells responded included insulin, transferrin, epidermal growth factor, thrombin, and somatomedins. The effect of thrombin did not require the availability of the active site of the protease. The effect of the somatomedins was only seen in the presence of heparin. Neither platelet-derived growth factor nor hydrocortisone induced arterial endothelial cell proliferation. These growth factor responses were also observed on the part of human umbilical vein endothelial cells.

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Citations

Aug 1, 1993·Calcified Tissue International·J G AndrewD Marsh
Apr 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·C F MoyerI Joris
Nov 11, 1992·Journal of the American College of Cardiology·R S SchwartzE J Topol
Jun 19, 2001·Journal of Immunological Methods·V MarinP Bongrand
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·E A StreetenM L Brandi
Jan 1, 1993·Growth Factors·P A D'Amore, S R Smith
Jan 1, 1997·Endothelium : Journal of Endothelial Cell Research·S BenvenutiM L Brandi
Jun 26, 1998·Journal of Cardiovascular Pharmacology·M R GralinskiM A Breider
Jul 9, 2020·Anatomia, histologia, embryologia·Juliane RiegerJohanna Plendl

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