Growth factor TGF-β induces intestinal epithelial cell (IEC-6) differentiation: miR-146b as a regulatory component in the negative feedback loop.

Genes & Nutrition
Yalin LiaoBo Lönnerdal

Abstract

TGF-β is a potent pleiotropic factor that promotes small intestinal cell differentiation. The role of microRNAs in the TGF-β induction of intestinal epithelial phenotype is largely unknown. We hypothesized that microRNAs are functionally involved in TGF-β-induced intestinal cell growth. In this study, TGF-β caused a morphological change of IEC-6 cells and stimulated expression of the epithelial cell markers alkaline phosphatase, villin, and aminopeptidase N. By global microRNA profiling during TGF-β-induced intestinal crypt cell (IEC-6) differentiation, we identified 19 differentially expressed microRNAs. We showed by real-time Q-PCR that miR-146b expression increased rapidly after TGF-β treatment; sequence analysis and in vitro assays revealed that miR-146b targets SIAH2, an E3 ubiquitin ligase, with decreased protein expression upon IEC-6 cell differentiation. Transfection of miR-146b inhibitor before TGF-β treatment blocked the down-regulation of SIAH2 in response to TGF-β. Moreover, SIAH2 over-expression during TGF-β treatment caused a significant decrease in Smad7 protein expression in IEC-6 cells. Furthermore, activation of the ERK1/2 pathway is active in the up-regulation of miR-146b by TGF-β. These findings suggest a no...Continue Reading

References

Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J A BarnardH L Moses
Mar 27, 2001·Nature Reviews. Molecular Cell Biology·A M Weissman
Dec 26, 2001·Nucleic Acids Research·Ron EdgarAlex E Lash
Jun 26, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tomoko HayashidaH William Schnaper
Aug 15, 2003·World Journal of Gastroenterology : WJG·Zhou WangJing-Bo Sun
Sep 4, 2003·American Journal of Physiology. Renal Physiology·Hisayo FujitaMidori Awazu
Dec 5, 2003·Oncogene·Antonia GermaniHeriberto Bruzzoni-Giovanelli
Mar 12, 2004·Genes & Development·John G Doench, Phillip A Sharp
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Elena SanchoHans Clevers
Feb 22, 2005·PLoS Biology·Julius BrenneckeStephen M Cohen
May 14, 2005·The Journal of Physiology·Georges DaoudJulie Lafond
Oct 22, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Fernand-Pierre GendronNathalie Rivard
Jun 24, 2006·Journal of Cellular Biochemistry·Sang-Won LeeHyun-Mo Ryoo
Feb 24, 2007·Current Opinion in Cell Biology·Susumu Itoh, Peter ten Dijke
Jun 13, 2008·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Jirina KolinskaBohuslav Dvorak
Jul 1, 2008·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Yao-Tseng ChenTheresa Scognamiglio
Sep 24, 2008·Annual Review of Physiology·Laurens G van der Flier, Hans Clevers
Mar 7, 2009·Brain Research·Hongping XiaMarie C Lin
Apr 1, 2009·Gastroenterology·Bon-Kyoung KooYoung-Yun Kong
Jun 13, 2009·FEBS Letters·Yosuke MizunoYasushi Okazaki
Jul 8, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Charles E RoglerLeslie E Rogler
Oct 27, 2009·Development·Kristi Wharton, Rik Derynck
Oct 27, 2009·Development·Aristidis Moustakas, Carl-Henrik Heldin
Nov 19, 2009·Cancer Research·Colin M HouseDavid D L Bowtell
Jan 22, 2010·Cells, Tissues, Organs·Fernando Rodríguez-SerranoAntonia Aránega
Feb 13, 2010·The Journal of Pediatrics·Irmeli A Penttila
Feb 13, 2010·The Journal of Pediatrics·Bo Lönnerdal
Apr 8, 2010·Cellular and Molecular Life Sciences : CMLS·Y Liao, B Lönnerdal

❮ Previous
Next ❯

Citations

May 24, 2013·Cell Biochemistry and Biophysics·Jianfei QiZe'ev A Ronai
Jan 12, 2016·Oncogene·C García-LimonesM A Calzado
Oct 25, 2016·The Journal of Nutritional Biochemistry·Man ZhangBo Lönnerdal
Dec 6, 2016·The Journal of Veterinary Medical Science·Hongyi LiEnqin Zheng
Feb 6, 2017·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Antoaneta Belcheva
Dec 19, 2017·Biotechnology and Bioengineering·Vincenza De GregorioPaolo A Netti

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.