Growth factors derived from a human malignant glioma cell line, U-251MG.

Journal of Neuro-oncology
J KuratsuG Y Gillespie

Abstract

A human malignant glioma cell line, U-251 Mg, cultured under serum free conditions, was shown to produce a growth factor for BALB/c 3T3 cells (glioma-derived growth factor-1, GDGF-1). The biological activity of GDGF-1 resided in a heat- and acid-resistant protein with a molecular weight (MW) of 25 kDa estimated by gel permeation chromatography. GDGF-1 activity was neutralized by a goat anti-human platelet derived growth factor (PDGF) antibody, indicating that the two factors were immunologically related. Furthermore, U-251 Mg cells constitutively expressed c-sis mRNA. When U-251 Mg cells were stimulated with bacterial lipopolysaccharide, 2 novel growth factors (GDGF-2 and GDGF-3) were produced in addition to the PDGF-like substance. GDGF-2 was determined to be greater than 100 kDa MW and was not neutralized by the goat anti-PDGF antiserum. The biological activity of GDGF-3 was also heat- and acid-resistant with an apparent 14 kDa MW. This factor also did not show any common antigenicity with PDGF. GDGF-2 and GDGF-3 are currently under investigation and evidence as to their natures will be published elsewhere. Our findings with this glioma cell line provide further evidence that inappropriate expression of growth factor-related ...Continue Reading

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Citations

Jan 1, 1995·Journal of Neuro-oncology·J KuratsuY Ushio
Feb 1, 1994·Hybridoma·E Lyon, G Y Gillespie
Jul 3, 2010·Journal of Neurosurgery. Pediatrics·Valerie N BartonRajeev Vibhakar
Sep 30, 1991·International Journal of Cancer. Journal International Du Cancer·E F AdamsR Fahlbusch

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