PMID: 2492022Jan 1, 1989Paper

Growth factors, signaling pathways, and the regulation of proliferation and differentiation in BC3H1 muscle cells. II. Two signaling pathways distinguished by pertussis toxin and a potential role for the ras oncogene

The Journal of Cell Biology
D J KelvinJ A Connolly

Abstract

In the preceding report (Kelvin, D.J., G. Simard, H.H. Tai, T.P. Yamaguchi, and J.A. Connolly. 1989. J. Cell Biol. 108:159-167) we demonstrated that pertussis toxin (PT) blocked proliferation and induced differentiation in BC3H1 muscle cells. In the present study, we have used PT to examine specific growth factor signaling pathways that may regulate these processes. Inhibition of [3H]thymidine by PT in 20% FBS was reversed in a dose-dependent fashion by purified fibroblast growth factor (FGF). In 0.5% FBS, the normally induced increase in creatine kinase (CK) activity was blocked by FGF in both the presence and absence of PT. Similar results were obtained with purified epidermal growth factor (EGF). We subsequently examined the effect of a family of growth factors linked to inositol lipid hydrolysis and found that thrombin, like FGF, would increase [3H]thymidine incorporation and block CK synthesis. However, PT blocked thymidine incorporation induced by thrombin, and blocked the inhibition of CK turn-on in 0.5% FBS by thrombin. The ras oncogene, a G protein homologue, has previously been shown to block muscle cell differentiation in C2 muscle cells (Olson, E.N., G. Spizz, and M.A. Tainsky. 1987. Mol. Cell. Biol. 7:2104-2111); w...Continue Reading

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Citations

Jun 1, 1992·Social Science & Medicine·D Mechanic
Jan 1, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·R E HausmanF Craft
Jan 1, 1996·The Journal of Cell Biology·S YoshidaY Nabeshima
Nov 16, 2007·Experimental Cell Research·Atsushi Mukai, Naohiro Hashimoto
Feb 1, 1989·Journal of Cellular Physiology·D J KelvinJ A Connolly
Jul 26, 2015·Applied Biochemistry and Biotechnology·Mohammed GagaouaMiguel Angel Sentandreu
Aug 3, 2014·Critical Reviews in Food Science and Nutrition·Yasmine BoudidaAhmed Ouali
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Jan 1, 2009·Progress in Molecular Biology and Translational Science·Hyeseon Cho, John H Kehrl
Jun 19, 2012·Free Radical Biology & Medicine·Jeong-Chae LeeXianglin Shi
May 12, 2015·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yunus AkkoçNarçin Palavan-Ünsal
Mar 20, 2016·Biochemical and Biophysical Research Communications·Yi ChenJinhui Wang
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Aug 1, 1990·Medical Hypotheses·M ChigiraH Watanabe

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