Growth hormone and insulin-like growth factor gene expression prior to the development of the pituitary gland in rainbow trout (Oncorhynchus mykiss) embryos reared at two temperatures

Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
Mao LiJohn Leatherland

Abstract

Real time RT-PCR was used to measure the changes in the rates of synthesis of mRNA encoding for growth hormone-1 (GH1) and -2 (GH2) and insulin-like growth factor-1 (IGF-1) and -2 (IGF-2), and whole embryo GH content was measured in early stage rainbow trout (Oncorhynchus mykiss) embryos reared at two incubation temperatures (8.5 and 6.0 degrees C). Particular attention was paid to the phase of embryo development that preceded the appearance of the pituitary gland. GH was present in zygotes, and there were no significant changes in whole embryo GH content of the two temperature treatment groups from fertilization (t0) until the time at which GH was detectable in the pituitary gland by immunostaining. The expression of the two GH genes decreased during the first 24 h post-fertilization, and then increased significantly by 17 dpf in embryos reared at both temperatures. There was a subsequent steep increase in the number of copies of GH1 and GH2 mRNA associated with the formation of the pituitary gland evident at 23 and 34 dpf in the 8.5 and 6.0 degrees C groups, respectively. The number of copies of mRNA encoding for IGF-1 and IGF-2 did not change during the first 24 h post-fertilization; however, there was a significant increase...Continue Reading

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Citations

Oct 26, 2010·Endocrine·S Harvey
Sep 24, 2013·General and Comparative Endocrinology·Laurence BesseauJack Falcón
Apr 22, 2014·General and Comparative Endocrinology·Steve Harvey, Marie-Laure Baudet
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