Growth hormone-dependent phosphorylation of tyrosine 333 and/or 338 of the growth hormone receptor.

The Journal of Biological Chemistry
J A VanderKuurC Carter-Su

Abstract

Many signaling pathways initiated by ligands that activate receptor tyrosine kinases have been shown to involve the binding of SH2 domain-containing proteins to specific phosphorylated tyrosines in the receptor. Although the receptor for growth hormone (GH) does not contain intrinsic tyrosine kinase activity, GH has recently been shown to promote the association of its receptor with JAK2 tyrosine kinase, to activate JAK2, and to promote the tyrosyl phosphorylation of both GH receptor (GHR) and JAK2. In this work, we examined whether tyrosines 333 and/or 338 in GHR are phosphorylated by JAK2 in response to GH. Tyrosines 333 and 338 in rat full-length (GHR1-638) and truncated (GHR1-454) receptor were replaced with phenylalanines and the mutated GHRs expressed in Chinese hamster ovary cells. These substitutions caused a loss of GH-dependent tyrosyl phosphorylation of truncated receptor and a reduction of GH-dependent phosphorylation of the full-length receptor. Consistent with Tyr333 and/or Tyr338 serving as substrates of JAK2, these substitutions resulted in a loss of tyrosyl phosphorylation of truncated receptor in an in vitro kinase assay using substantially purified GH.GHR.JAK2 complexes. The Tyr to Phe substitutions did not s...Continue Reading

References

Nov 5, 1978·Journal of Molecular Biology·J Janin, S Wodak
Oct 30, 1992·Cell·T Pawson, G D Gish
Oct 22, 1990·Molecular and Cellular Endocrinology·T E AdamsM R Brandon
Dec 1, 1990·Molecular Endocrinology·H SadeghiW R Baumbach
Apr 20, 1990·Cell·A Ullrich, J Schlessinger
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·N BillestrupJ H Nielsen
Nov 11, 1990·Nucleic Acids Research·J A CioffiJ J Kopchick
Sep 10, 1990·Molecular and Cellular Endocrinology·S D HauserG G Krivi
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Mar 31, 1995·The Journal of Biological Chemistry·J VanderKuurC Carter-Su
Mar 12, 1993·Cell·Z SongyangR J Lechleider
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·O SilvennoinenJ N Ihle
Jul 1, 1993·Current Biology : CB·T Pawson, J Schlessingert

❮ Previous
Next ❯

Citations

Jan 6, 2000·General and Comparative Endocrinology·L F JacksonC V Sullivan
Oct 29, 2008·General and Comparative Endocrinology·Ana Lupe Motta StudzinskiLuis Fernando Marins
Nov 21, 2013·Investigational New Drugs·Doris Mangiaracina BenbrookK Darrell Berlin
Mar 1, 2006·Expert Review of Endocrinology & Metabolism·Rebecca A Pelekanos, Michael J Waters
Jul 1, 2008·Expert Review of Endocrinology & Metabolism·Louise T DalgaardJens H Nielsen
Aug 16, 1996·The Journal of Biological Chemistry·B C XuJ J Kopchick
Dec 10, 1999·The Journal of Biological Chemistry·P A Ram, D J Waxman
Oct 19, 2002·Critical Reviews in Clinical Laboratory Sciences·Paul Shapiro
Nov 18, 2000·American Journal of Physiology. Cell Physiology·P L BergadS A Berry
Oct 9, 2007·The Journal of Biological Chemistry·Iwona PileckaRob Hooft van Huijsduijnen
Sep 11, 2001·Biochemical and Biophysical Research Communications·S LiuG F Hu

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Related Papers

Toxicology and Applied Pharmacology
Jonathan M Shipley, D J Waxman
Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas
S C Crepaldi-AlvesA C Boschero
© 2021 Meta ULC. All rights reserved