Growth hormone differentially regulates muscle myostatin1 and -2 and increases circulating cortisol in rainbow trout (Oncorhynchus mykiss)

General and Comparative Endocrinology
P R BigaTroy L Ott

Abstract

Myostatin (MSTN) negatively regulates muscle growth in vertebrates. Salmonids produce two myostatin transcripts from separate genes. Surprisingly, quantitative analyses indicate different regulatory mechanisms for the two myostatin genes in rainbow trout. MSTN1 mRNA levels were elevated 26% following recombinant bovine growth hormone (rbGH) treatment, while MSTN2 mRNA levels were reduced 74% compared to controls. MSTN precursor protein (42kDa) levels were elevated in rbGH treated fish compared to controls. In addition, circulating cortisol levels were elevated 71% following rbGH treatment compared to controls. In treated and control fish, cortisol levels were elevated 245% at day 0 compared to subsequent days. Treated fish exhibited cortisol levels 207% higher than controls at 0.5 day, and remained at least 50% higher for 7 days following treatment. This pattern of change was positively correlated to MSTN1 mRNA levels. This is the first time a direct relationship has been reported between GH, cortisol, and myostatin. In addition, following rbGH administration, myosin protein concentrations in skeletal muscle samples increased, suggesting that GH regulates expression of the most abundant muscle protein. These results indicate th...Continue Reading

References

Oct 1, 1985·General and Comparative Endocrinology·R S NishiokaH A Bern
Feb 5, 1968·Annals of the New York Academy of Sciences·J L Kostyo
Nov 14, 1997·Proceedings of the National Academy of Sciences of the United States of America·A C McPherron, S J Lee
Jul 27, 2000·Journal of Cellular Physiology·S KirkJ Bass
Sep 8, 2000·The Journal of Biological Chemistry·M ThomasR Kambadur
Feb 13, 2001·American Journal of Physiology. Endocrinology and Metabolism·W E TaylorN Gonzalez-Cadavid
Jun 12, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·B D Rodgers, G M Weber
Aug 2, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C H LangR A Frost
Oct 2, 2001·Molecular Endocrinology·A M DelanyE Canalis
Oct 19, 2001·European Journal of Biochemistry·T K Østbye Andersen Ø
Nov 10, 2001·American Journal of Physiology. Endocrinology and Metabolism·K MaS Bhasin
Nov 10, 2001·American Journal of Physiology. Endocrinology and Metabolism·T J MarcellM R Blackman
Jan 15, 2003·The Journal of Endocrinology·S VianelloL Colombo
Feb 22, 2003·Cell and Tissue Research·Giuseppe RadaelliBruria Funkenstein
Apr 5, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Buel D RodgersMichael A Levine
May 2, 2003·American Journal of Physiology. Endocrinology and Metabolism·Kun MaBehrouz Salehian
Oct 14, 2003·The Journal of Experimental Biology·Cheng XuShao-Jun Du
Nov 7, 2003·The Journal of Clinical Endocrinology and Metabolism·Wei LiuShereen Ezzat
Nov 15, 2003·Molecular and Cellular Endocrinology·Steven B Roberts, Frederick W Goetz

❮ Previous
Next ❯

Citations

Apr 25, 2013·In Vitro Cellular & Developmental Biology. Animal·Jacob Michael FroehlichPeggy R Biga
May 31, 2014·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Nicholas J GaltPeggy R Biga
Feb 22, 2012·Acta Physiologica·B ElliottR Mackenzie
Jul 4, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Katherine A JohansenKen Overturf
Jun 24, 2006·Experimental Cell Research·Dominique Joulia-EkazaCabello Gérard
Jun 25, 2013·General and Comparative Endocrinology·Eduardo N FuentesBjörn Thrandur Björnsson
Feb 7, 2015·Science China. Life Sciences·XiangYan DaiZhan Yin
Sep 14, 2007·DNA Sequence : the Journal of DNA Sequencing and Mapping·Liangyi XueLu Li
May 29, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Erika F MedeirosTerence M Bradley
Jul 15, 2015·Polish Journal of Veterinary Sciences·A K DevrimM Sudagidan
Apr 15, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Iban SeiliezJean-Charles Gabillard
Aug 6, 2009·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Peggy R Biga, Jessica Meyer

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.