Growth hormone (GH)-induced dimerization inhibits phorbol ester-stimulated GH receptor proteolysis

The Journal of Biological Chemistry
Y ZhangStuart J Frank

Abstract

Growth hormone (GH) initiates its cellular action by properly dimerizing GH receptor (GHR). A substantial fraction of circulating GH is complexed with a high-affinity GH-binding protein (GHBP) that in many species can be generated by GHR proteolysis and shedding of the receptor's ligand-binding extracellular domain. We previously showed that this proteolysis 1) can be acutely promoted by the phorbol ester phorbol 12-myristate 13-acetate (PMA), 2) requires a metalloprotease activity, 3) generates both shed GHBP and a membrane-associated GHR transmembrane/cytoplasmic domain remnant, and 4) results in down-regulation of GHR abundance and GH signaling. Using cell culture model systems, we now explore the effects of GH treatment on inducible GHR proteolysis and GHBP shedding. In human IM-9 lymphocytes, which endogenously express GHRs, and in Chinese hamster ovary cells heterologously expressing wild-type or cytoplasmic domain internal deletion mutant rabbit GHRs, brief exposure to GH inhibited PMA-induced GHR proteolysis (receptor loss and remnant accumulation) by 60-93%. PMA-induced shedding of GHBP from Chinese hamster ovary transfectants was also inhibited by 70% in the presence of GH. The capacity of GH to inhibit inducible GHR ...Continue Reading

References

Jan 1, 1992·Hormone Research·T AmitZ Hochberg
Jul 1, 1991·The Journal of Clinical Endocrinology and Metabolism·P M MarthaG Baumann
Jul 1, 1991·The Journal of Clinical Endocrinology and Metabolism·D A MannorG Baumann
Dec 1, 1990·Molecular Endocrinology·H SadeghiW R Baumbach
Jul 1, 1989·Metabolism: Clinical and Experimental·G BaumannK Amburn
Aug 29, 1986·Biochemical and Biophysical Research Communications·A C HeringtonJ L Stevenson
Jun 16, 1995·The Journal of Biological Chemistry·S J FrankA S Kraft
Jun 1, 1995·Molecular and Cellular Endocrinology·P E MullisP M Brickell
Oct 20, 1995·The Journal of Biological Chemistry·V P KnutsonM Lopez-Reyes
Apr 1, 1994·The Journal of Endocrinology·G Baumann
Mar 8, 1996·The Journal of Biological Chemistry·Y HanG R Stark
Jan 1, 1996·Annual Review of Physiology·C Carter-SuL S Smit
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·F DastotS Amselem
Feb 1, 1997·Molecular and Cellular Biology·F KohlhuberI M Kerr
Apr 1, 1997·The Journal of Endocrinology·R Barnard, M J Waters
Jan 27, 1998·The Journal of Biological Chemistry·S O KimS J Frank

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Citations

Jan 10, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Farrah Kheradmand, Zena Werb
Feb 2, 2008·Cellular and Molecular Life Sciences : CMLS·A J Beel, C R Sanders
Jun 1, 2005·Experimental and Molecular Pathology·Marc FrémontPatrick Englebienne
Mar 31, 2012·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Peter D PioliJohn J Krolewski
Oct 6, 2010·The Journal of Clinical Investigation·Mahendra D MavalliThomas L Clemens
Nov 30, 2012·Molecular Endocrinology·Jie XuStuart J Frank
Jan 26, 2013·Molecular Endocrinology·Edward O ListJohn J Kopchick
Apr 4, 2002·Biological Chemistry·Persio Dello Sbarba, Elisabetta Rovida
Jul 9, 2002·Journal of Pediatric Endocrinology & Metabolism : JPEM·Graciela Piwien-PilipukJessica Schwartz
Jan 12, 2002·Biochemical and Biophysical Research Communications·Françoise ConteEric Bieth
Feb 19, 2013·Endocrinology·Ryan M CorrickJoseph L Messina
Mar 17, 2007·FEBS Letters·Elena BurovaNikolai Nikolsky
Dec 19, 2012·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Carolina S MartinezAna I Sotelo
Dec 22, 2009·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Johanna G MiquetAna I Sotelo
Oct 13, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Panos G ZirosAthanasios G Papavassiliou
Apr 25, 2006·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Jens Fuglsang, Per Ovesen
Dec 20, 2005·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Sanne Fisker
Nov 17, 2009·Biochemical and Biophysical Research Communications·Ning YangStuart J Frank
Jul 1, 2008·Biochimica Et Biophysica Acta·Stuart J Frank, Serge Y Fuchs
Feb 19, 2015·Journal of Molecular Endocrinology·Carolina S MartinezAna I Sotelo
Apr 26, 2011·Biochemical and Biophysical Research Communications·Xiangdong WangStuart J Frank
Dec 18, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Matthew A HeldLee A Denson
Mar 4, 2005·The Journal of Biological Chemistry·Jon W CowanStuart J Frank
Feb 28, 2002·Endocrinology·Váronique BeauloyeJean-Paul Thissen
Mar 19, 2002·Endocrinology·Peter van KerkhofGer J Strous
Feb 11, 2003·Molecular Endocrinology·Jürgen GentGer J Strous
Feb 3, 2007·Endocrinology·Gaddameedi R ReddyRam K Menon
Sep 5, 2014·Molecular Endocrinology·Ying LiuStuart J Frank
Jun 5, 2014·American Journal of Physiology. Endocrinology and Metabolism·Yueshui ZhaoYin Xia

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