PMID: 7580939Jan 1, 1995Paper

Growth hormone (GH)-induced tyrosine-phosphorylated proteins in cells that express GH receptors

Receptor
P A HardingJ J Kopchick

Abstract

We have shown previously that growth hormone (GH)-induced tyrosine phosphorylation of a 95-kDa protein in mouse L-cells stably transfected with the GH receptor. In addition to induction of pp95, we have established that GH also induces tyrosine phosphorylation of a 42-kDa protein and a 130-kDa protein, as detected with phosphotyrosine antibodies. A time course of tyrosine phosphorylation on GH treatment indicates that within the GH signal transduction cascade, tyrosine phosphorylation of pp95 occurs by 1 min, whereas tyrosine phosphorylation of pp42 was not detected until 5 min. Additionally, the concentration of GH needed to stimulate tyrosine phosphorylation of pp42 was greater than that required for pp95. The pp42 protein comigrates with a 42-kDa protein identified as extracellular signal-regulated kinase 2 (ERK2). Growth factors, such as FGF, PDGF, IGF-I, and insulin, induce tyrosine phosphorylation of pp42 in pGHR-W10 cells and in 3T3-F442A preadipocytes; however, they are unable to induce pp95. These results suggest that GH induction of tyrosine-phosphorylated pp42 may represent a common signal transduction point of various growth factors, including GH, whereas tyrosine phosphorylation of pp95 is GH specific.

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