PMID: 8967451May 1, 1996Paper

Growth hormone increases calcium uptake in rat fat cells by a mechanism dependent on protein kinase C

The American Journal of Physiology
S GaurH M Goodman

Abstract

Growth hormone (GH; 500 ng/ml) rapidly doubled cytosolic free Ca2+ concentration ([Ca2+]i) in rat adipocytes as determined with the Ca2+ indicator fura 2. No response was seen in Ca(2+)-free medium, suggesting that the increase in [Ca2+]i was due to Ca2+ influx. GH also doubled the influx of Mn2- as inferred from the rate of fluorescence quenching. Depolarization with 30 mMK+ also increased [Ca2+]i, and the increase in [Ca2+]i due to either GH or 30 mMK+ was blocked by 100 nM nimodipine, suggesting that GH increases [Ca2+]i by activating voltage-sensitive L-type Ca2+ channels. GH increased [Ca2+]i even when K+ channels were blocked, suggesting that activation of Ca2+ uptake was not secondary to closure of K+ channels and consequent depolarization. A diacylglycerol (PAG) analogue, 1,2-dioctanoyl-sn-glycerol (50 microM), duplicated, and the protein kinase C(PKC) inhibitors calphostin C (100 nM), chelerythrine (1 microM), and bis-indolylmaleimide (250 nM) inhibited the effects of GH on [Ca2+]i. Xanthogenate tricyclodecan-9-yl (D609), a specific inhibitor of phospholipase C(PLC), abolished the increase in [Ca2+]i due to GH but not to DAG. The results suggest that GH increases [Ca2+]i by activation of PLC, release of DAG, and activa...Continue Reading

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Citations

Aug 4, 1998·The American Journal of Physiology·S GaurH M Goodman
Jul 9, 2002·Journal of Pediatric Endocrinology & Metabolism : JPEM·Graciela Piwien-PilipukJessica Schwartz
Aug 17, 1999·Annual Review of Nutrition·J J KopchickK T Coschigano
Aug 28, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Philippe G Cammisotto, Ludwik J Bukowiecki
Apr 5, 2000·The Journal of Biological Chemistry·A SjöholmP O Berggren
Jul 18, 2002·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·S Y Nam, P E Lobie
May 29, 2014·American Journal of Physiology. Endocrinology and Metabolism·Donna C BentleyPaul A Smith
Aug 10, 2001·Cellular Signalling·T ZhuP E Lobie
May 5, 2004·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·I Marrero, A Déniz

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