PMID: 8967450May 1, 1996Paper

Growth hormone regulates cytosolic free calcium in rat fat cells by maintaining L-type calcium channels

The American Journal of Physiology
S GaurH M Goodman

Abstract

In freshly isolated individual rat adipocytes, cytosolic free Ca2+ concentration ([Ca2+]i) as measured with fura 2 slowly declined during incubation but was sustained, or even somewhat increased, by brief treatment with growth hormone (GH) at the beginning of a 3-h incubation period. GH-treated adipocytes were more permeable to Ca2+ than GH-deprived cells as indicated, using Mn2- as a surrogate and monitoring influx by the rate of quenching of fura 2 fluorescence. Blockage of Ca2- channels with 100 nM nimodipine lowered [Ca2+]i in GH-treated cells to the level seen in GH-deprived cells. Increases in [Ca2+]i or the rate of Mn2+ entry were twofold greater in GH-treated than in GH-deprived cells when extracellular K+ was increased to 30 mM. Similarly, the Ca2+ channel agonist BAY K 5552 or the diacylglycerol analogue 1,2-dioctanoyl-sn-glycerol increased [Ca2+]i more in GH-treated than in GH-deprived adipocytes. Ca(2+)-ATPase activity was two times higher in plasma membranes isolated from GH-treated than from GH-deprived cells. Continued synthesis of Ca(2+)-ATPase may depend on [Ca2+]i, since the effects of GH on [Ca2+]i and Ca(2+)-ATPase were blocked by a cycloheximide or verapamil. We suggest that voltage-sensitive L-type Ca2+ ch...Continue Reading

References

May 1, 1992·Endocrinology·P E LobieM J Waters

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Citations

Apr 5, 2000·The Journal of Biological Chemistry·A SjöholmP O Berggren
May 12, 2010·American Journal of Physiology. Cell Physiology·Atsushi OguriToshiaki Nakajima
Mar 17, 2020·Adipocyte·Mingzhu ZhaiWuping Sun
Aug 4, 1998·The American Journal of Physiology·S GaurH M Goodman
Jul 9, 2002·Journal of Pediatric Endocrinology & Metabolism : JPEM·Graciela Piwien-PilipukJessica Schwartz
Jan 18, 2018·The Biochemical Journal·Mickaël F El HachmaneCharlotta S Olofsson
Jan 12, 1999·Archives of Biochemistry and Biophysics·M YorekK Bielefeldt

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